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首页> 外文期刊>African Journal of Biotechnology >Production of cellulolytic and xylanolytic enzymes by a phytopathogenic Myrothecium roridum and some avirulent fungal isolates from water hyacinth
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Production of cellulolytic and xylanolytic enzymes by a phytopathogenic Myrothecium roridum and some avirulent fungal isolates from water hyacinth

机译：致植物病原性的Myrothecium roridum和一些来自风信子的无毒真菌分离物产生的纤维素分解酶和木糖分解酶

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摘要

The cellulolytic and xylanolytic activity of a pathogenic?Myrothecium roridum?Tode (IMI 394934) and non-pathogenic?Fusarium solani?and?Curvularia pallescenceBoedjin isolates from water hyacinth were investigated. The mycelial plugs of each isolate was grown in submerged cultures of Czapeck Dox broth containing the appropriate carbon source (carboxymethylcellulose, sawdust and homogenized dry water hyacinth leaf) at 25°C for 16 days. The enzyme activity assay was carried out on the culture filtrates obtained. This was measured as micromole sugar released per min. The result obtained showed that the enzyme activity (U/ml) for?b-1,4-exoglucanase,?b-1,4-endoglucanase and xylanase was maximum 3.70?±?0.43, 0.95±?0.03 and 2.32?±?0.10, respectively, in?C. pallescens?Boedjin grown on carboxymethylcellulose and minimum 0.12 ± 0.02, 0.13 ± 0.03 and 0.34 ± 0.01 respectively, in?M. roridum?grown on homogenized dry water hyacinth leaf. The?b-glucosidase activity (U/ml) was highest, 1.74?±?0.06 in?M. roridum?grown on sawdust and least, 0.08 ± 0.00 in?C.?pallescens?Boedjin grown on homogenized water hyacinth leaf broth. The maximum (324.00 ± 19.51?mg/ml) and minimum (130.00 ± 5.83?mg/ml) total extracellular protein was produced in?M. roridum?grown on homogenized dry water hyacinth leaf and carboxymethylcellulose, respectively. This study showed that the phytopathogenic strain of?M. roridum?is capable of producing cellulases and xylanase enzyme in submerged cultures but to a lesser degree compared to?F. solani?and?C. pallescence?Boedjin.
机译：研究了从风信子中分离出的致病性罗氏沼霉Tode（IMI 394934）和非致病性日疫镰刀菌和枯萎菌Boedjin分离株的纤维素分解和木糖分解活性。将每个分离物的菌丝体塞在含有适当碳源（羧甲基纤维素，锯末和均化的干水葫芦叶）的Czapeck Dox肉汤的水下培养物中于25°C培养16天。对获得的培养物滤液进行酶活性测定。测量为每分钟释放的微摩尔糖。得到的结果表明，αb-1，4-外切葡聚糖酶，βb-1，4-内葡聚糖酶和木聚糖酶的酶活性（U / ml）最大为3.70±0.34、0.95±0.03和2.32±3.2。分别为0.10in.C。 pallescens？Boedjin生长在羧甲基纤维素上，最小分别为0.12±0.02、0.13±0.03和0.34±0.01 in？M。 roridum？生长在均质的干风信子叶子上。 αb-葡糖苷酶活性（U / ml）最高，为1.74±0.06in·M。锯末生长在木屑上，至少在纯水葫芦叶肉汤上生长0.08±0.00 in.C.pallescens？Boedjin。在？M中产生最大（324.00±19.51？mg / ml）和最小（130.00±5.83？mg / ml）总细胞外蛋白。种植在均质的干风信子叶和羧甲基纤维素上这项研究表明，致病性菌株是？M。腐殖质能够在淹没的培养物中产生纤维素酶和木聚糖酶，但与ΔF相比，其程度较低。 solani和C。枯萎了吗？

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