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首页> 外文期刊>African Journal of Biotechnology >Association of single nucleotide polymorphisms in IGF-I, IGF-II and IGFBP-II with production traits in breeder hens of Mazandaran native fowls breeding station
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Association of single nucleotide polymorphisms in IGF-I, IGF-II and IGFBP-II with production traits in breeder hens of Mazandaran native fowls breeding station

机译：IGF-I，IGF-II和IGFBP-II中单核苷酸多态性与Mazandaran本地家禽繁殖站种鸡的生产性状的关联

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The purpose of this study was to detect the polymorphism in IGF-I, IGF-II and IGFBP-II marker loci and their association with body weight at 8 weeks, average egg weight and total number of eggs laid during first 12 weeks after flocks maturity in breeder hens of native fowls. Blood samples were collected randomly from 160 individuals and genomic DNA was extracted using modified salting out method. A set of specific primer pairs were used for amplification of target genomic DNA at each marker loci and polymorphisms were detected using PCR-RFLP method. For IGF-I and IGFBP-II marker loci, allele B was the most frequent allele and ranged from 0.61 to 0.63 while, allele A was identified as a dominant allele in IGF-I marker site due to the highest frequency (0.57). The frequency of AA homozygous genotype was the lowest among all marker loci (0.08), whereas, AB genotype showed the highest frequency (0.61). Analysis of phenotypic data showed that the average egg weight and total number of eggs laid during first 12 weeks after flocks maturity were significantly affected by IGF-II and IGF-I marker loci respectively. No significant associations were observed between IGFBP-II genotypes and production traits. Comparison between detected alleles in the present study with reported allele by other research groups revealed a new allelic pattern for the analyzed marker loci in breeder hens of Mazandaran native fowls breeding station.
机译：这项研究的目的是检测IGF-I，IGF-II和IGFBP-II标记基因座的多态性，以及它们与鸡群在8周时的体重，平均蛋重和成年后头12周产蛋总数的关系。在本地家禽的种鸡中。随机收集160名个体的血样，并使用改良的盐析法提取基因组DNA。使用一组特异性引物对在每个标记位点扩增靶基因组DNA，并使用PCR-RFLP方法检测多态性。对于IGF-I和IGFBP-II标记基因座，等位基因B是频率最高的等位基因，范围为0.61至0.63，而由于频率最高（0.57），等位基因A被鉴定为IGF-I标记位点的优势等位基因。在所有标记基因座中，AA纯合基因型频率最低（0.08），而AB基因型显示最高频率（0.61）。对表型数据的分析表明，鸡群成熟后前12周的平均蛋重和产蛋总数分别受到IGF-II和IGF-I标记基因座的显着影响。没有观察到IGFBP-II基因型与生产性状之间的显着关联。本研究中检测到的等位基因与其他研究小组报告的等位基因之间的比较揭示了Mazandaran本地家禽繁殖站种鸡的分析标记位点的新等位基因模式。

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