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Effect of melatonin on in vitro maturation of bovine oocytes

机译：褪黑素对牛卵母细胞体外成熟的影响

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To investigate the effect of different concentrations of melatonin on bovine oocytesin vitro?maturation, varying concentrations of melatonin (0, 0.01, 1, 100 μM), were included in the the maturation medium. Slaughterhouse derived oocytes were subjected to standard?in vitro?maturation procedures in high oxygen tension. After culture for 24 h, over 80% of COCs had full cumulus cells expansion. As melatonin concentration increased, the degree of cumulus cells expansion did not changed significantly. Oocytes incubated in 0.01 and 1 μM melatonin-containing media for 24 h, result in 73.11 and 70.68% maturation rate, respectively, which were no different from the control (72.24%). The maturation rate decreased (P < 0.05) significantly when melatonin concentrations was increased from 0.01 to 1 and 100 μM (73.11 vs 70.68% and 65.24% respectively). After culture in melatonin-containing media for 24 h, 100% oocytes achieved GVBD stage in all groups. There were no significant differences among groups at GVBD stage. But oocytes that remained at metaphase-I stage were significant (P < 0.05) different with 100 μM in compare to other groups (21.32 Vs 17.67, 15.68, 16.53).?In conclusion in this experiment,melatonin cannot improve cumulus cell expansion and nuclear maturation of bovine oocytes. When concentrations is high, melatonin may affect bovine oocytes meiotic maturation at metaphase-1 stage, but it is improbable melatonin be toxic for bovine oocytes.
机译：为了研究不同浓度的褪黑激素对牛卵母细胞体外成熟的影响，在成熟培养基中加入了不同浓度的褪黑激素（0、0.01、1、100μM）。屠宰场衍生的卵母细胞在高氧张力下进行标准的体外成熟程序。培养24小时后，超过80％的COC具有完全的卵丘细胞扩增。随着褪黑激素浓度的增加，卵丘细胞的扩张程度没有明显改变。卵母细胞在含有0.01和1μM褪黑素的培养基中孵育24小时，分别达到73.11和70.68％的成熟率，与对照组（72.24％）没有差异。当褪黑激素浓度从0.01增加到1和100μM时，成熟率显着降低（P <0.05）（分别为73.11对70.68％和65.24％）。在含褪黑激素的培养基中培养24小时后，所有组中100％的卵母细胞均达到GVBD阶段。 GVBD阶段各组之间无显着差异。但是，与其他组相比，处于I期中期的卵母细胞与100μM组相比有显着差异（P <0.05）（21.32 Vs 17.67，15.68，16.53）。牛卵母细胞的成熟。当浓度高时，褪黑激素可能会影响中期1阶段牛卵母细胞的减数分裂成熟，但褪黑素对牛卵母细胞的毒性是不可能的。

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