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首页> 外文期刊>African Journal of Biotechnology >Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers
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Micropropagation of some Malaysian banana and plantain (Musa sp.) cultivars using male flowers

机译：使用雄花对某些马来西亚香蕉和车前草（Musa sp。）品种进行微繁

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Male inflorescences have potential to be used as explants for rapid micropropagation of?Musa?sp. The male flowers of four banana cultivars, namely ‘Berangan’, ‘Rastali’, ‘Nangka’ and ‘Abu’ belonging to three genome types in?Musa(AAA, AAB, and ABB), were cultured onto Murashige and Skoog (MS) medium which was supplemented with 1 mg/L of TDZ, BAP, Kin, 2-ip and Zea. The number of shoots was found to significantly increase in both TDZ and BAP treatments, as compared to other cytokinins. TDZ at 0.4, 0.6 and 0.8 mg/L, in particular, appeared to be optimum for shoot induction in ‘Berangan-AAA’, ‘Rastali-AAB’ and ‘Nangka-AAB’ and ‘Abu-ABB’, respectively. However, all the cultivars showed their highest response to regeneration at 8 mg/L of BAP. After the initiation of the explants onto the MS media for all the cultivars, the highest number of cauliflower-like bodies’ (CLBs) clusters was observed at two months of culture. The number of induced ‘CLBs’ cluster is dependent on the size of male buds. Male inflorescences with the size of 20 mm were found to induce more ‘CLBs’ clusters. Meanwhile, the number of shoots produced is dependent on both the cytokinins and cultivars used.
机译：雄性花序有潜力用作外来体以快速微繁殖穆萨氏菌。在Musashi（AAA，AAB和ABB）的三个基因组类型中，将四个香蕉品种'Berangan'，'Rastali'，'Nangka'和'Abu'的雄花培养到Murashige和Skoog（MS）上培养基，其中添加了1 mg / L TDZ，BAP，Kin，2-ip和Zea。与其他细胞分裂素相比，TDZ和BAP处理的芽数均显着增加。 TDZ分别为0.4、0.6和0.8 mg / L似乎最适合分别在“ Berangan-AAA”，“ Rastali-AAB”和“ Nangka-AAB”和“ Abu-ABB”中诱导芽生。然而，在8 mg / L的BAP下，所有品种对再生的反应最高。在将所有品种的外植体接种到MS培养基上后，在培养两个月后观察到了最多的花椰菜样小体（CLB）簇。诱导的“ CLB”簇的数量取决于雄芽的大小。发现大小为20 mm的雄性花序诱导更多的“ CLB”簇。同时，产生的芽的数量取决于所使用的细胞分裂素和品种。

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