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首页> 外文期刊>African Journal of Biotechnology >Optimization of callus induction and plant regeneration from germinating seeds of sweet sorghum (Sorghum bicolor Moench)
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Optimization of callus induction and plant regeneration from germinating seeds of sweet sorghum (Sorghum bicolor Moench)

机译：甜高粱发芽种子愈伤组织的诱导和植物再生的优化

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摘要

An efficient regeneration system was developed using germinating seeds of two cultivars of sweet sorghum,?Sorghum bicolor?'Yuantian No.1' and 'M81E', as explants. We tested different media supplements effects on callus induction.?The effects of combinations of 2,4-D, KT, sucrose, agar and proline at different concentrations on callus induction were compared. While 'Yuantian No.1' seeds showed the highest embryogenic callus induction frequency (57%) on MS medium supplemented with 4 mg l-1?2,4-D, 0.2 mg l-1?KT, 0.6 g l-1?proline, 45 g l-1?sucrose and 0.8 g l-1?agar, 'M81E' seeds showed highest embryogenic callus induction frequency (74%) on MS medium supplemented with 4 mg l-1?2,4-D, 0.2 mg l-1?KT, 45 g l-1?sucrose and 1.2 g l-1?agar. The best shoot induction was observed when?theexplants were cultured on MS medium supplemented with 1 mg l-1?IAA and 3 mg l-16-BA, and on MS medium supplemented with 2 mg l-1?6-BA and 0.5 mg l-1?KT for 'Yuantian No.1' and 'M81E', respectively. The regenerated shoots were transferred onto MS medium supplemented with 3 mg l-1?IBA for rooting. After 2 weeks of culture, the percentage of root production was more than 85%. The plantlets were hardened on 1/2 MS medium and then transplanted to pots,?where they?exhibited morphologically normal growth.
机译：利用两个甜高粱“双色高粱”“圆田1号”和“ M81E”两个品种的发芽种子作为外植体，开发了一种高效的再生系统。我们测试了不同培养基补充剂对愈伤组织诱导的影响。比较了2，4-D，KT，蔗糖，琼脂和脯氨酸的组合在不同浓度下对愈伤组织诱导的影响。而“元田一号”种子在补充了4 mg l-1？2,4-D，0.2 mg l-1？KT，0.6 g l-1？的MS培养基上显示出最高的胚性愈伤组织诱导频率（57％）。脯氨酸，45 g l-1？蔗糖和0.8 g l-1？琼脂，'M81E'种子在补充4 mg l-1？2,4-D，0.2的MS培养基上显示出最高的胚性愈伤组织诱导频率（74％）毫克l-1？KT，45克l-1？蔗糖和1.2 g l-1？琼脂。当外植体在补充有1 mg l-1？IAA和3 mg l-16-BA的MS培养基上以及在补充有2 mg l-1？6-6-BA和0.5 mg的MS培养基上培养时，观察到最佳芽诱导。 l-1？KT分别代表“圆田1号”和“ M81E”。将再生的芽转移到补充有3mgI-1βIBA的MS培养基上生根。培养2周后，根系产生的百分比超过85％。将小苗在1/2 MS培养基上硬化，然后移植到盆中，在那里表现出形态上正常的生长。

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