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首页> 外文期刊>African Journal of Biotechnology >Polyploidy induction of Tunisian Trigonella foenum-greaum L. populations
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Polyploidy induction of Tunisian Trigonella foenum-greaum L. populations

机译：突尼斯Trigonella foenum-Greaum L.种群的多倍体诱导

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摘要

Polyploidy plays an important role in plant evolution and constitutes an important mechanism of diversification and creation of genetic variability. Artificial polyploidy can be induced using the colchicine. The aim of this?study was to determine theTrigonella foenum-greacum?(2n= 16) population which resists better to colchicine treatment and which has the highest rate of polyploid formation among 38 populations collected from different Tunisian regions. The comparative study between treated and control plants showed significant increases (p < 0.05) for the parameters survival rate, rate of malformed leaves appearance, stem height and seeds number by pod. The 38 treated populations showed significant variations (p < 0.05) between them for the parameters survival rate, branches number, stem height and seeds number by pod. Results also showed that treatment of the shoot meristem by colchicine allowed to obtain diploid (2n= 16) and mixoploid plants having mixoploid branches (4n = 32 and 2n= 16) and branches entirely tetraploid (4n = 32). The obtained rate of mixoploidy was 65.79% of treated plants. Among the 38 analyzed fenugreek populations, the population 15 of Menzel Temime presented the highest rate of mixoploids formation and survival rate to colchicine treatment.
机译：多倍体在植物进化中起着重要作用，并构成了多样化和遗传变异创造的重要机制。可以使用秋水仙碱诱导人工多倍体。这项研究的目的是确定在不同突尼斯地区收集的38个种群中，对秋水仙碱的抗药性更好，多倍体形成率最高的Trigonella foenum-greacum（2n = 16）种群。处理植物和对照植物之间的比较研究表明，参数存活率，叶片畸形率，茎高和荚果种子数参数显着提高（p <0.05）。 38个处理过的种群之间的参数存活率，枝数，茎高和荚果种子数之间存在显着差异（p <0.05）。结果还表明，秋水仙碱处理芽分生组织可以得到二倍体（2n = 16）和具有混合倍体分支（4n = 32和2n = 16）且分支完全为四倍体（4n = 32）的混合倍体。获得的混合倍性率为处理植物的65.79％。在38个分析的胡芦巴种群中，Menzel Temime种群中的15个在秋水仙碱治疗中表现出最高的混合倍体形成率和存活率。

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