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首页> 外文期刊>African Journal of Biotechnology >In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)
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In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroideae: Orchidaceae)

机译：从巨大的蝴蝶兰原形体（PLB）和愈伤组织（Epidendroideae：Orchidaceae）再生离体植物

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摘要

Phalaenopsis, with long arching sprays of flowers, are among the most beautiful flowers in the world.?Phalaenopsis?is an important genus and one of the most popular epiphytic monopodial orchids, grown commercially for?the?production of cut flowers and potted plants. Most of them have different and interesting morphological characteristics which have different value to the breeders.?Phalaenopsis gigantea?is one of the most difficult to grow and has the potential of producing beautiful hybrids. An efficient and reproducible method for large-scale propagation of?Ph. giganteausing leaf sections has been developed. Leaf sections?from?in vitro?young plants were cultured on New Dogashima Medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), Thidiazuron (TDZ), and Kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mg/L) alone and in combinations with (auxins a-naphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mg/L). The explants developed calli and protocorm-like-bodies (PLBs) within 6 weeks of culture. Treatment TDZ in combination with auxins was found to be?the?best for the induction of callus and PLBs.?In vitroregeneration of?Ph.?gigantea?PLB was achieved by exposure to light and transferring to hormone free NDM solid medium.
机译：蝴蝶兰长有拱形的花朵，是世界上最美丽的花朵之一。蝴蝶兰是重要的属，也是最受欢迎的附生单足兰花之一，商业上种植以生产切花和盆栽植物。它们中的大多数具有不同且有趣的形态特征，这些特征对育种者具有不同的价值。蝴蝶兰是最难生长的植物之一，具有产生美丽杂种的潜力。一种有效且可重现的大规模传播Ph的方法。已开发出可用于巨型植物的叶片。在添加了细胞分裂素（6-苄基氨基嘌呤（BAP），噻二唑隆（TDZ）和激动素（KIN））的新八a岛培养基（NDM）上培养离体幼树的叶片，分别以0.01、0.1、0.5和1.0毫克/升）单独使用或与（生长素α-萘乙酸（NAA）以0.01、0.1、0.5和1.0毫克/升结合使用）。外植体在培养的6周内形成了愈伤组织和原球茎样体（PLB）。已发现将TDZ与植物生长素结合使用是“诱导愈伤组织和PLB的最佳方法”。巨藻PhAB的体外再生是通过暴露于光并将其转移至无激素的NDM固体培养基来实现的。

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