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首页> 外文期刊>African Journal of Biotechnology >Generation and analysis of cDNA library from lipopolysaccharide-stimulated gastropod abalone (Haliotis diversicolor supertexta) hemocytes
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Generation and analysis of cDNA library from lipopolysaccharide-stimulated gastropod abalone (Haliotis diversicolor supertexta) hemocytes

机译：脂多糖刺激的腹足类鲍鱼（Haliotis diversicolor supertexta）血细胞的cDNA文库的产生和分析

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A cDNA Library of lipopolysaccharide-stimulated gastropod abalone hemocytes was constructed using switching mechanism at 5' end of RNA transcript (SMART) approach. 300?random clones were selected and sequenced.?180 ESTs was gained.?After assembling, we obtained 24 contigs and 119 singlets finally, some of which were immune- related genes. These immune-related genes include cytidine deaminase, ferritin, nonmuscle myosin essential light chain, cytochrome c oxidase subunit I, CD63 antigen-like protein and lysosomal-associated transmembrane protein. This study may contribute?to the understanding of the immune mechanism of gastropod abalone?Haliotis diversicolor supertexta.
机译：利用RNA转录本（SMART）方法的5'末端的转换机制，构建了脂多糖刺激的腹足类鲍鱼血细胞的cDNA文库。选择了300个随机克隆并进行了测序。获得了180个EST。组装后，我们最终获得了24个重叠群和119个单峰，其中一些是与免疫相关的基因。这些与免疫相关的基因包括胞苷脱氨酶，铁蛋白，非肌肉肌球蛋白必需轻链，细胞色素C氧化酶亚基I，CD63抗原样蛋白和溶酶体相关跨膜蛋白。这项研究可能有助于了解腹足类鲍鱼的免疫机制。

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