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首页> 外文期刊>African Journal of Biotechnology >Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica)
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Cloning and heterologous expression of a gene encoding lycopene-epsilon-cyclase, a precursor of lutein in tea (Camellia sinensis var assamica)

机译：茶中叶黄素前体番茄红素-ε-环化酶编码基因的克隆和异源表达

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摘要

This report describes the cloning and expression of a gene lycopene epsilon cyclase,?(LCYE) from?Camellia sinensis?var?assamica?which is a precursor of the carotenoid lutein in tea. The?1982 bp cDNA sequence with 1599 bp open reading frame of LCYE?was identified from an SSH library constructed for quality trait in tea. 5’ and 3’ RACE?(rapid-amplification of cDNA ends) was done to clone the full length cDNA of LCYE. Homology studies showed that the deduced amino acid sequence of LCYE gene had the highest sequence identity of up to 84% with?Vitis vinefera. The cloned gene was successfully expressed in a PET based?Escherichia coliexpression system. The size of the expressed protein?was?59615 Daltons.?A suppression subtractive library was constructed using a quality clone H3111 (tester) and a garden series clone T3E3 (driver).
机译：该报告描述了茶树中类胡萝卜素叶黄素的前体“茶树”中番茄红素ε环化酶（LCYE）的克隆和表达。从构建用于茶品质性状的SSH文库中鉴定出具有1599bp开放阅读框的LCYEα的1982bp cDNA序列。进行5’和3’RACE？（cDNA末端的快速扩增）以克隆LCYE的全长cDNA。同源性研究表明，推导的LCYE基因的氨基酸序列与葡萄藤具有最高的序列同一性，最高可达84％。克隆的基因在基于PET的大肠杆菌表达系统中成功表达。表达的蛋白质的大小为59615道尔顿。使用优质克隆H3111（测试仪）和花园系列克隆T3E3（驱动剂）构建抑制消减文库。

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