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首页> 外文期刊>African Journal of Biotechnology >Seed germination and in vitro regeneration of the African medicinal and pesticidal plant, Bobgunnia madagascariensis
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Seed germination and in vitro regeneration of the African medicinal and pesticidal plant, Bobgunnia madagascariensis

机译：非洲药用和杀虫植物Bobgunnia madagascariensis的种子发芽和离体再生

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摘要

Propagation of the medicinal and pesticidal tree,?Bobgunnia madagascarensis?is difficult due to poor and erratic germination of its seeds and slow growth of its seedlings. This study involved two separate experiments. The first evaluated the effect of pre-sowing treatments and growing medium on?ex vitro?seed germination and early seedling development. The second experiment involved?in vitrogermination, shoot initiation and rooting of shoots. Pre-sowing seed treatments involved soaking seeds in cold and hot water for 12 and 24 h and soaking in different concentrations (0, 100, 200, 400 and 800 mg/l) of gibberellic acid for 24 h. Soaking of seeds in cold or hot water for up to 24 h did not achieve more than 45% germination, while seeds treated with gibberellic acid achieved 76%) when seeds were sown in a growing medium without compost compared with a medium with compost (<43%). All shoot-tips isolated from the?in vitro?germinated seedlings on B5 media without plant growth regulators continued to grow as a single shoot, while shoot-tips cultured on B5 supplemented with 0.1 mg/l of naphthaleneacetic acid (NAA) and thidiazuron (TDZ) produced two shoots each after four weeks. It was concluded that?B. madagascariensis?seeds had very low?ex vitro?germination percentages. Although,?in vitro?cultures improved seed germination, axillary shoot multiplication and rooting were not satisfactory.?Therefore, further studies are needed to develop an optimal?in vitro?multiplication protocol for?B. madagascariensis.
机译：由于其种子发芽差且不稳定且幼苗生长缓慢，药用和农药树（“ Bobgunnia madagascarensis”）的繁殖非常困难。这项研究涉及两个单独的实验。第一个评估了播前处理和生长培养基对离体种子萌发和幼苗早期发育的影响。第二个实验涉及体外发芽，芽的萌芽和生根。播前种子处理包括将种子在冷热水中浸泡12和24小时，然后以不同浓度（0、100、200、400和800 mg / l）的赤霉素浸泡24小时。将种子播种在没有堆肥的生长培养基中（与有堆肥的培养基相比），将种子在冷水或热水中浸泡24小时达不到发芽率的45％，而用赤霉素处理的种子播种率达到76％。 43％）。在没有植物生长调节剂的情况下，在B5培养基上从萌发的幼苗中分离出的所有芽尖均继续单芽生长，而在补充有0.1 mg / l萘乙酸（NAA）和噻二唑的B5上培养的芽尖（ TDZ）在四个星期后各产生了两个芽。结论是？马达加斯加种子在体外的发芽率非常低。尽管体外培养可以改善种子的发芽，但腋芽的繁殖和生根效果却不令人满意。因此，仍需要进一步的研究，以开发出一种针对B的最佳体外繁殖方案。马达加斯加半岛。

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