• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Cloning and mRNA expression pattern analysis under low temperature stress of EAAP gene in Dongmu-70 rye
【24h】

Cloning and mRNA expression pattern analysis under low temperature stress of EAAP gene in Dongmu-70 rye

机译：东牧70黑麦EAAP基因低温胁迫下的克隆及mRNA表达模式分析

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This research cloned endochitinase-antifreeze protein precursor?(EAPP) gene of Dong-mu 70 rye (Secale cereale) by designing special primers according to Genbank’s?EAPP?gene sequence, and analyzing the influence of low temperature stress on the expression of mRNA with RT-PCR. The results indicated that theEAPP’s full length coding of Dong-mu-70 rye was 1085 bp, while the homology of its amino acid and that of?Hordeum vulgare?and?Triticum aestivum?was much higher (95 and 91%, respectively). The mRNA of the?EAPP?gene in Dong-mu 70 rye was expressed in roots under normal temperature, and was most active in the leaves. When stressed at a low temperature of 4°C, remarkable improvements were shown in mRNA expression in the root and stems, and the maximum expression appeared 24 h after treatment and decreased afterwards.
机译：本研究通过根据Genbank的“ EAPP”基因序列设计特殊引物，克隆了冬木70黑麦的内切几丁质内切酶-抗冻蛋白前体基因（EAPP），并分析了低温胁迫对mRNA表达的影响。 RT-PCR。结果表明，EAPP的Dong-mu-70黑麦的全长编码为1085 bp，而其氨基酸与“大麦”和“普通小麦”的氨基酸同源性更高（分别为95％和91％）。东牧70黑麦中“ EAPP”基因的mRNA在常温下在根中表达，并且在叶片中最活跃。当在4°C的低温下胁迫时，根和茎中的mRNA表达显示出显着的改善，最大表达出现在处理后24小时，随后下降。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号