• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Impact of genotype, age of tree and environmental temperature on androgenesis induction of Aesculus hippocastanum L.
【24h】

Impact of genotype, age of tree and environmental temperature on androgenesis induction of Aesculus hippocastanum L.

机译：基因型，树龄和环境温度对欧洲七叶树雌激素诱导的影响。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Influence of ten different genotypes, age of trees and environmental temperature?on induction of androgenesis and apperance albino horse chestnut embryos were studied. Efficiency of?in vitro?androgenesis?via?anther and microspore culture had been investigated. Microspores and anthers were used from the same closed flower bud. Androgenic response of different genotypes was measured and compared. Anther induction rates were from 5 to 37.6%, depending on genotype. The number of embryos per isolated anther varied between 0.5 to 5.0 embryos in anther culture, while in microspore culture varied between 3.0 to 27 embryos, depending on genotype. A?microspore culture was 5 - 6 times efficient than anther culture for same genotype.?Age of the trees had no influence on androgenesis induction.?Temperature of about 4 - 5°C was optimal for androgenic embryo induction.?Albino horse chestnut embryos?phenomenon?depended on genotype. The number of albino appearing in anther was higher than in microspore culture. The same correlation of appearing albino was observed between short and long day.Flow cytogenetic analysis of androgenic embryos originating from anther and microspore culture was done after a first generation of regenerants. All androgenic embryos, the first generation from microspore culture were haploid, while 50% of the regenerants originating from anther culture were haploid, and the other half diploid.
机译：研究了十种不同基因型，树龄和环境温度对诱导雄激素和外观白化七叶树胚的影响。已经研究了通过花药和小孢子培养的体外雄激素生成效率。小孢子和花药从相同的闭合花芽中使用。测量和比较了不同基因型的雄激素反应。取决于基因型，花药诱导率为5-37.6％。在每个花药培养物中，每个分离的花药的胚数在0.5到5.0个胚之间变化，而在小孢子培养中，在3.0到27个胚之间变化，这取决于基因型。对于相同基因型，小孢子培养的效率是花药培养的5-6倍。树木的年龄对雄激素的诱导没有影响。温度约4-5°C对于雄性胚的诱导是最佳的。现象取决于基因型。花药中出现的白化病数量高于小孢子培养。在短日和长日之间观察到相同的出现白化病的相关性。在第一代再生剂之后，对源自花药和小孢子培养的雄性胚进行流式细胞遗传学分析。来自小孢子培养的第一代所有雄激素胚均为单倍体，而源自花药培养的再生体的50％为单倍体，另一半为二倍体。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号