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首页> 外文期刊>African Journal of Biotechnology >Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme from Beauveria bassiana isolates
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Effects of carbon and nitrogen sources on the induction and repression of chitinase enzyme from Beauveria bassiana isolates

机译：碳和氮源对球孢白僵菌分离株几丁质酶诱导和抑制的影响

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摘要

Beauveria bassiana?a natural soil borne insect pathogen is being used effectively these days in integrated pest management system.?Foliar application of these fungi is quite satisfactory as it invades its host by adhering to insect cuticles through the formation of penetration structures called appresoria, which produces various extracellular enzymes, including chitinase that causes the insect cuticle breaching. Although many investigations have been done in this regard, only a little is known about the induction and repression mechanism of this hydrolytic enzyme. This report illustrates the effect of two carbon sources; colloidal chitin and dextrose and a nitrogen source, yeast extract on the chitinase production of seventeen?B. bassiana?isolates. The chitinase activity varied among the isolates and the different media studied. A high enzymatic activity was observed in the medium with colloidal chitin as a sole source of carbon followed by the medium containing an extra nitrogen?source, yeast extract. Exochitinase activity and the chitinase activity gel were also determined for the isolates showing high chitinase enzyme activity. An array of chitinase isozymes were observed on chitinase activity gel with a common 70 kDa enzyme for all the isolates.?Keywords:?Beauveria bassiana, induction, colloidal chitin, chitinase, exochitinase.
机译：球孢白僵菌（Beauveria bassiana）是一种天然的土壤传播的昆虫病原体，目前已在综合害虫管理系统中得到有效利用。这些真菌的叶面施用非常满意，因为它通过形成称为阑尾的穿透结构而粘附在昆虫表皮上，从而侵入了宿主。产生各种细胞外酶，包括几丁质酶，这些酶会导致昆虫表皮破裂。尽管已经在这方面进行了许多研究，但是关于这种水解酶的诱导和抑制机理了解很少。该报告说明了两种碳源的影响。胶质几丁质和右旋糖和一个氮源，酵母提取物对几丁质酶的产量为十七？B。低音孤立。几丁质酶活性在分离物和所研究的不同培养基之间变化。在以胶体甲壳质为唯一碳源的培养基中，然后是含有额外氮源的酵母提取物的培养基中，观察到了很高的酶活性。还确定了具有高几丁质酶活性的分离物的外切酶活性和几丁质酶活性凝胶。在几丁质酶活性凝胶上观察到一系列的几丁质酶同工酶，所有分离物均具有共同的70 kDa酶。

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