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首页> 外文期刊>African Journal of Biotechnology >Optimization of micropropagation and establishment of cell suspension culture in Melissa officinalis L.
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Optimization of micropropagation and establishment of cell suspension culture in Melissa officinalis L.

机译：蜜蜂（Melissa officinalis L）的微繁优化和细胞悬浮培养的建立。

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Melissa officinalis?L.,?due to its useful application in medicine, is being paid more attention. In order to establish a stable regeneration system with 4 landraces collected from different climate in Iran, major parameters such as regeneration rate, rooting percentage, shooting induction, proliferation rate, fresh and dry weight as a biomass of cells were investigated. Statistical analysis of results showed that BAP in combination with NAA had the highest regeneration in shoot tips explants. NAA in combination with IAA and kinetin had the best response to callus induction. Also 1 mgl/l NAA had a higher response to rooting than other auxins used. 2,4-D at 1.0 mg/l and BAP at 0.5 mg/l showed the highest production of fresh and dry weight, 5.48 and 0.407 g, respectively, that is approximately 20 times the initial weight of callus. 2,4-D (1 mg/l) and BAP (0.5 mg/l) had the highest cells number.
机译：蜜蜂花由于其在医学上的有用应用而受到越来越多的关注。为了建立一个稳定的再生系统，利用伊朗不同气候收集的4个地方品种，研究了主要参数，如再生速率，生根率，射击诱导，增殖速率，鲜重和干重作为细胞生物量。结果的统计分析表明，BAP与NAA结合在芽尖外植体中再生最高。 NAA与IAA和激动素的结合对愈伤组织诱导的反应最佳。 1 mgl / l NAA对生根的反应也比所用的其他生长素高。 1.0 mg / l的2,4-D和0.5 mg / l的BAP分别显示最高的鲜重和干重，分别为5.48和0.407 g，大约是愈伤组织初始重量的20倍。 2,4-D（1 mg / l）和BAP（0.5 mg / l）的细胞数最高。

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