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The comparison of immunomodulatory effects of peripheral mononuclear cells against proliferation in U937 in junior elderly habitual morning swimming in Taiwan cohort

机译:台湾人群初中老年人惯常游泳中外周血单个核细胞对U937增殖的免疫调节作用比较

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The present investigation was carried out to evaluate the effect of greater immunomodulatory effects of human peripheral blood mononuclear cells against proliferation in human leukemia cells.? To achieve this, cells U937 in junior elderly (with cool environmental physical activities) subjects with habitual morning swimming and sedentary lifestyle were recruited in relatively cool season in Taiwan; the isolated human peripheral blood mononuclear cells were stimulated by phytohemagglutinin to obtain the conditioned medium which contains various cytokines. However, the differential effects of the conditioned medium on growth inhibition in U937 leukemia cells were observed. The cytokines, including interferon-gamma, tumor necrosis factors-alpha and interleukine-2 secreted into conditioned medium were higher in the morning-swimming subjects than in the sedentary-lifestyle ones. Similarly, serum white blood cell, creatine phosphokinase,immunoglobulin G?and?immunoglobulin A?in the morning-swimming and sedentary-lifestyle groups indicated that no further inflammatory status existed in the morning-swimming group. In summary, greater immunomodulatory effects of human peripheral blood mononuclear cells against proliferation in human leukemia cells U937 in junior elderly subjects came from the effects of regular moderate exercise in cool temperature rather than of inflammatory effects.
机译:进行本研究以评估人外周血单核细胞对人白血病细胞增殖的更大免疫调节作用的作用。为此,在台湾相对凉爽的季节中,招募了习惯于早晨游泳和久坐的生活方式的初中老年人(从事凉爽的环境体育锻炼)的U937细胞。用植物血凝素刺激分离的人外周血单核细胞,得到含有各种细胞因子的条件培养基。但是,观察到条件培养基对U937白血病细胞生长抑制的不同作用。早晨游泳的受试者中,分泌到条件培养基中的细胞因子(包括干扰素-γ,肿瘤坏死因子-α和白细胞因子2)要比久坐的生活方式高。类似地,晨泳和久坐生活方式组的血清白细胞,肌酸磷酸激酶,免疫球蛋白Gα和“免疫球蛋白A”表明,晨泳组没有进一步的炎症状态。总之,在老年老年人中,人外周血单核细胞对人白血病细胞U937增殖的更大免疫调节作用来自于在凉爽的温度下进行定期适度运动而不是发炎的作用。

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