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首页> 外文期刊>African Journal of Biotechnology >Genome-wide detection of Ty1-copia and Ty3-gypsy group retrotransposons in Japanese apricot (Prunus mume Sieb. et Zucc.)
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Genome-wide detection of Ty1-copia and Ty3-gypsy group retrotransposons in Japanese apricot (Prunus mume Sieb. et Zucc.)

机译：全基因组检测日本杏中的Ty1-copia和Ty3-gypsy组逆转座子（李子Sieb。et Zucc。）

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The?conserved domains of reverse transcriptase (RT) genes of approximately 260 bp for?Ty1-copia?and 430 bp for?Ty3-gypsy?groups of long terminal repeat (LTR) retrotransposons were amplified from Japanese apricot?(Prunus mume?Sieb. et Zucc.)?using degenerate oligonucleotide primers. Sequence analysis showed that 32.3% of?Ty1-copia?and 27.5% of?Ty3-gypsy?RT sequences possessed stop codons and/or frameshifts, and all sequences were AT-rich.?Ty1-copiaretrotransposon has higher heterogeneity than?Ty3-gypsy?retrotransposon, but the latter has a higher copy number revealed by southern dot blot hybridization. Phylogenetic analysis illustrated that some of the clones were more closely related to the representative elements present in other plant species than to other clones of Japanese apricot. Transcription was not detected by reverse transcription-polymerase chain reaction amplification for either?Ty1-copia?or?Ty3-gypsyretrotransposons in young leaves of plants treated with UV light and 2,4-dichlorophenoxyacetic acid either individually or in both combinations, even though the ratios of dN/dS of the open reading frames among members of each subgroup of both group retrotransposons were less than 1. This is the ?rst report on the presence of RT sequences of?Ty1-copia?and?Ty3-gypsy?group retrotransposons in Japanese apricot genome.
机译：从日本杏（Prunus mume？Sieb）扩增长末端重复（LTR）反转录转座子的“ Ty1-copia”组约260 bp的逆转录酶（RT）基因保守域和“ Ty3-gypsy”组约430 bp。 （等）。使用简并的​​寡核苷酸引物。序列分析表明，32.3％的“ Ty1-copia”序列和27.5％的“ Ty3-gypsy” RT序列具有终止密码子和/或移码，并且所有序列均富含AT。ΔTy1-copiaretrotransposon的异质性高于ΔTy3-。吉普赛逆转录转座子，但后者具有更高的拷贝数，这是通过Southern dot blot杂交揭示的。系统发育分析表明，某些克隆与其他植物物种中存在的代表性元素比与日本杏的其他克隆更紧密相关。逆转录-聚合酶链反应扩增未检测到单独或以两种组合方式用紫外线和2,4-二氯苯氧基乙酸处理过的植物幼叶中的Ty1-copiaβ或Ty3-gypsyretrotransposons的转录。两组逆转录转座子的每个亚组成员之间的开放阅读框的dN / dS之比均小于1。这是关于“ Ty1-copia”和“ Ty3-gypsy”群逆转座子RT序列存在的首次报道。在日本杏基因组中。

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