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Response surface methodology for optimization of the treatment of textile wastewater by a novel bacterial consortium: Enzymes and metabolites characterization

机译:用响应面方法优化新型细菌财团对纺织废水的处理:酶和代谢产物表征

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Textile wastewater (TWW) causes many environmental problems, and colored substances must be removed from it before their discharge into the environment. An optimization process was analyzed using custom response surface methodology (RSM). In the present study, the effect of different combinations of eight strains was studied using equilateral triangle diagram (Minitab 14.0) and mixture experimental design to assess color and?chemical oxygen demand?(COD) removal during the growth of species. Under optimal conditions, the bacterial consortium developed was able to decolorize completely (>84%) the dye within 72 h. The color and COD removal were 89.97 and 84.71%, respectively. A significant increase in azoreductase, lignin peroxidase and laccase activities in the cells were obtained after complete decolorization.?Ultraviolet (UV)–vis and nuclear magnetic resonance (NMR) spectroscopy analysis confirmed the biodegradability of the TWW by the developed bacterial consortium.
机译:纺织废水(TWW)引起许多环境问题,在将有色物质排放到环境中之前,必须将其清除。使用自定义响应面方法(RSM)分析了优化过程。在本研究中,使用等边三角形图(Minitab 14.0)和混合实验设计来研究八个菌株的不同组合的效果,以评估物种生长过程中颜色和“化学需氧量”(COD)的去除。在最佳条件下,开发的细菌财团能够在72小时内使染料完全脱色(> 84%)。去除颜色和化学需氧量分别为89.97%和84.71%。完全脱色后,细胞中的偶氮还原酶,木质素过氧化物酶和漆酶活性显着增加。紫外线(UV)-可见光和核磁共振(NMR)光谱分析证实了发达的细菌财团对TWW的生物降解能力。

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