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首页> 外文期刊>African Journal of Biotechnology >Effects of calpastain (CAST) polymorphisms on carcass and meat quality traits in Mongcai pigs
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Effects of calpastain (CAST) polymorphisms on carcass and meat quality traits in Mongcai pigs

机译：钙蛋白酶（CAST）多态性对旺财仔猪car体和肉品质性状的影响

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Calpastain (CAST) activity plays a major role in muscle growth and proteolytic changes post-mortem and the CAST gene has been considered as a candidate gene for carcass and pork quality characteristics. The aim of this study was to analyze the association of two polymorphisms namely CAST_HinfI (allele A and B) and CAST_MspI (allele C and D) with carcass and meat quality traits in Mongcai, a Vietnamese indigenous pig breed. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to genotype the animals at these loci. Results indicate that the CAST_HinfI?single nucleotide polymorphism (SNP)?had a low frequency of allele A as compared to allele B, while the C and D allele distribution was almost the same for the CAST_MspI SNP. In the association analysis, significant effects on dressing percentage of carcass were detected. The CAST_HinfI locus was associated with the pH24, while the CAST_MspI position was in association with pH45 min, drip loss48?and redness color. Additional analysis showed a variation in muscle fiber type composition with higher proportion of IIx fiber in pigs with AB genotype (P?< 0.05). Three constructed haplotypes namely AB/CD, AB/DD and BB/CC also had significant effects on carcass, type IIa and IIb fiber percentages.
机译：钙蛋白酶（CAST）活性在死后肌肉生长和蛋白水解变化中起主要作用，并且CAST基因已被认为是car体和猪肉品质特征的候选基因。这项研究的目的是分析越南本地猪品种旺财的两个多态性，即CAST_HinfI（等位基因A和B）和CAST_MspI（等位基因C和D）与car体和肉质性状的关联。聚合酶链反应-限制性片段长度多态性（PCR-RFLP）被用于对这些基因座处的动物进行基因分型。结果表明，与等位基因B相比，CAST_HinfI单核苷酸多态性（SNP）的等位基因A的频率较低，而CAST_MspI SNP的C和D等位基因分布几乎相同。在关联分析中，检测到对car体修整百分比的显着影响。 CAST_HinfI位点与pH24相关，而CAST_MspI位置与pH45 min，滴水损失48？和红色相关。进一步的分析表明，在具有AB基因型的猪中，肌肉纤维类型组成的变化与IIx纤维的比例更高（P <0.05）。 AB / CD，AB / DD和BB / CC这三种构建的单倍型也对car体，IIa型和IIb型纤维百分比具有显着影响。

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