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首页> 外文期刊>African Journal of Biotechnology >Relationships among alfalfa resistance to Sclerotinia crown and stem rot, Sclerotinia trifoliorum and oxalic acid
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Relationships among alfalfa resistance to Sclerotinia crown and stem rot, Sclerotinia trifoliorum and oxalic acid

机译：苜蓿对菌核菌冠和茎腐病，菌核菌和草酸的抗性关系

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摘要

Sclerotinia crown and stem rot (SCSR) of alfalfa caused by?Sclerotinia trifoliorum?is one of the main constraints for efficient alfalfa cultivation in temperate climate all over the world. The resistance of 200 alfalfa accessions to Sclerotinia crown and stem rot was evaluated during 2010 to 2011 in the field nursery established in 2009. The resistance of alfalfa accessions germinating seeds to mycelium of?Sclerotinia trifoliorum?and oxalic acid (OA) concentrations of 10, 20, 30 mM was screened under laboratory conditions. The statistically significant differences at?P<0.05?were determined among evaluated alfalfa accessions resistance to all screened factors. The reactions of alfalfa accessions to disease under field conditions showed that majority of the non-adapted accessions were heavily diseased, whereas the resistant accessions had only negligible disease severity. The germination of accessions seeds at oxalic acid concentration of 30 mM showed strong correlation (r = -0.817**,P<0.01) with SCSR severity in 2011. Among OA concentrations, this one showed the highest correlation rate with SCSR as well as was the least time consuming method. This method of seeds germination on mycelium of?S. trifoliorum?was unfit due to its weak correlation with SCSR and higher inputs.
机译：苜蓿菌核病菌（Sclerotinia trifoliorum）引起的苜蓿菌核菌冠和茎腐病（SCSR）是在全球温带气候下有效进行苜蓿栽培的主要限制因素之一。在2009年建立的田间苗圃中，于2010年至2011年评估了200个紫花苜蓿种质对菌核菌冠和茎腐病的抗性。发芽的紫花苜蓿种质对三叶菌核盘菌和草酸（OA）浓度为10，在实验室条件下筛选了20、30 mM。在评估的苜蓿种质对所有筛选因子的抗性之间，在ΔP＜0.05处确定了统计学上的显着差异。苜蓿种在田间条件下对疾病的反应表明，大多数不适应的种均病情严重，而抗性种仅病情严重程度可忽略不计。草酸浓度为30 mM的种质种子的萌发与2011年的SCSR严重程度呈极强的相关性（r = -0.817 **，P <0.01）。在OA浓度中，该品种与SCSR的相关性最高，且最省时的方法。这种种子在？S菌丝体上发芽的方法。 trifoliorum？不适合，因为它与SCSR的相关性较弱，且投入较高。

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