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首页> 外文期刊>African Journal of Biotechnology >Embryogenesis and plant regeneration from unpollinated ovaries of Amorphophallus konjac
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Embryogenesis and plant regeneration from unpollinated ovaries of Amorphophallus konjac

机译：魔芋未授粉卵巢的胚发生和植物再生

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The system of somatic embryogenesis of?Amorphophallus konjac?had been built through unpollinated ovaries. The embryogenic calli were induced on?Murashige and Skoog (MS)?basal medium supplemented with 9.0 μM 6- benzylaminopurine?(BA), 0.4 μM 2,4- dichlorophenoxyacetic acid?(D), 1.0 μM naphthaleneacetic acid?(NAA), and the induction rate was 34.0%. The differentiation rate was 35.5% on the medium of MS basal medium supplemented with 6.7 μM 6-BA and 2.2 μM NAA. The obtained plantlets were transferred into rooting medium which was 1/2MS supplementing with 2.7 μM NAA, and the rooting rate was above 95%. All of the media were added 3% (w/v) sucrose and 0.3% (w/v) phytagel, the experimental materials for each step were cultured at 25 ± 2°C with a photoperiod of 12 h and light intensity of 50 μmol m-2?s-1.
机译：魔芋魔芋的体细胞胚发生系统是通过未授粉的卵巢构建的。在补充了9.0μM6-苄氨基嘌呤（BA），0.4μM2,4-二氯苯氧基乙酸（D），1.0μM萘乙酸（NAA）的Murashige和Skoog（MS）基础培养基上诱导胚性愈伤组织，诱导率为34.0％。在补充了6.7μM6-BA和2.2μMNAA的MS基础培养基上，分化率为35.5％。将获得的小植株转移到生根培养基中，生根培养基为1 / 2MS，添加2.7μMNAA，生根率高于95％。所有培养基均添加3％（w / v）蔗糖和0.3％（w / v）phytagel，每一步的实验材料在25±2°C下以12 h的光周期和50μmol的光强度培养m-2？s-1。

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