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首页> 外文期刊>African Journal of Biotechnology >Serological and molecular detection and prevalence of Cucurbit aphid-borne yellows virus in the Sistan region, Iran
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Serological and molecular detection and prevalence of Cucurbit aphid-borne yellows virus in the Sistan region, Iran

机译：伊朗锡斯坦地区葫芦科蚜虫传播的黄病毒的血清学和分子检测及流行

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During the 2009 growing seasons, virus-like symptoms were noticed on cucurbit crops (melons (Cucumis melo?L.) and watermelons [Citrullus lanatus?(Thunb.) Matsum and Nakai)] grown in the Sistan region. The symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thickening of older leaves. 100 melon samples from symptomatic plants from 32 melon fields were collected for virus detection during 2009 and 2010. In a preliminary serological study, extracts of diseased leaves did not react with antisera to viruses reported from area, namely?Cucumber mosaic virus,?Watermelon mosaic virus-2 and?Zucchini yellow mosaic virus. During the entire survey,?CABYV?was detected by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) in all samples. Analysis of these samples were done with reverse transcription-polymerase chain reactions with a pair of primers designed to amplify the coat protein region of?CABYV?which resulted in the expected product (approximately 479 bp), confirming that all melon samples were infected by?CABYV. The high incidence of?CABYV?in infections suggests that this virus may well become an important threat for cucurbit crops in the Sistan region.
机译：在2009年的生长季节中，在锡斯坦地区生长的葫芦类作物（瓜类（Cucumis melo？L。）和西瓜[Citrullus lanatus？（Thunb。Matsum and Nakai）]]上发现了类似病毒的症状。症状很普遍，包括最初的褪绿病损害，然后是全叶变黄和老叶变厚。在2009年至2010年期间，从32个甜瓜田的有症状植物中收集了100个甜瓜样品用于病毒检测。在一项血清学初步研究中，患病叶片的提取物与抗血清对当地报道的病毒没有反应，即黄瓜花叶病毒，西瓜花叶病毒。病毒2和西葫芦黄色花叶病毒。在整个调查过程中，所有样品均通过双抗体夹心酶联免疫吸附测定（DAS-ELISA）检测到了“ CABYV”。这些样品的分析是通过逆转录-聚合酶链反应，用一对设计成能扩增“ CABYV”外壳蛋白区域的引物进行的，从而产生了预期的产物（约479 bp），证实所有瓜类样品都被？ CABYV。 “ CABYV”感染的高发生率表明该病毒很可能成为锡斯坦地区葫芦科作物的重要威胁。

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