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首页> 外文期刊>African Journal of Biotechnology >Complete genomic sequence and recombination analysis of wheat yellow mosaic virus isolate from Zhouzhi in China
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Complete genomic sequence and recombination analysis of wheat yellow mosaic virus isolate from Zhouzhi in China

机译：中国周至小麦黄花叶病毒分离株的完整基因组序列和重组分析

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摘要

Wheat yellow mosaic virus (WYMV) is the causal agent of wheat yellow mosaic disease in China. WYMV was detected in wheat sample collected from Zhouzhi of Shanxi province. The nearly complete genomic sequence of Zhouzhi isolate (WYMV-ZZ) was determined; it was compared with six complete sequences of WYMV isolates (five Chinese isolates and one Japanese isolate). WYMV-ZZ and the other six different WYMV isolates shared 96.6 to 97.7% and 95.1 to 98.2% nucleotide sequence identity for RNA1 and RNA2, respectively; at the amino acid level, WYMV-ZZ had 94.1 to 98.2% identity for RNA1 and 94.1 to 96.7% identity for RNA2, respectively, with the other six isolates. Phylogenetic analysis showed that the NIa-VPg region can separate the Chinese isolates from Japanese isolate. Based on the recombinant analysis, there were three possible recombination events; one event was located in RNA1 CI region of WYMV-ZZ with a RDP P-value of 8.526×10-06. This work advanced our understanding of the WYMV molecular variation and was helpful to study the disease spread.
机译：小麦黄花叶病毒（WYMV）是中国小麦黄花叶病的病原体。从山西周至采集的小麦样品中检测到WYMV。确定了周至分离株（WYMV-ZZ）的近乎完整的基因组序列;将其与WYMV分离株的6个完整序列（5个中国分离株和1个日本分离株）进行了比较。 WYMV-ZZ和其他六个不同的WYMV分离株分别具有RNA1和RNA2的96.6％至97.7％和95.1％至98.2％的核苷酸序列同一性。在氨基酸水平上，WYMV-ZZ与其他六个分离株分别具有与RNA1相同的94.1％至98.2％和与RNA2相同的94.1％至96.7％。系统发育分析表明，NIa-VPg区可以将日本分离株与中国分离株区分开。根据重组分析，可能发生三种重组事件。一个事件位于WYMV-ZZ的RNA1 CI区，RDP P值为8.526×10-06。这项工作增进了我们对WYMV分子变异的了解，并有助于研究疾病的传播。

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