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首页> 外文期刊>African Journal of Biotechnology >Evaluation of proteases produced by Erwinia chrysanthemi for the deproteinization of crustacean waste in a chitin production process
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Evaluation of proteases produced by Erwinia chrysanthemi for the deproteinization of crustacean waste in a chitin production process

机译：菊花欧文氏菌产生的蛋白酶对甲壳素生产过程中甲壳类废物脱蛋白的评估

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This study evaluated the potential use of proteases produced by?Erwinia chrysanthemi?for the extraction of pure chitin from crustacean wastes using standard methods. The crude bacterial protease had activity of 22.4 U/ml. Protein removal by the commercial protease were similar in both the mineralized and demineralized wastes and amounted to about 75 and 80%, respectively, in 16 h. A similar trend was observed with higher protein removal efficiency (95%) for the crude protease from both the mineralized and demineralized wastes. Also, treatment of the?powder with 5% NaOH resulted in the removal of protein in the demineralized and mineralized wastes decreased by about 96 and 87.6%, respectively, in same period from an initial concentration of 0.3 mg/ml.?It was concluded that proteases produced by?E. chrysanthemi?could be used to transform waste from sea-food processing industries into products of commercial value.
机译：这项研究评估了欧文氏菊（Erwinia chrysanthemi）产生的蛋白酶在使用标准方法从甲壳类废物中提取纯甲壳质中的潜在用途。粗细菌蛋白酶的活性为22.4U / ml。通过商业蛋白酶去除的蛋白质在矿化和矿物质废物中相似，在16小时内分别达到约75％和80％。在矿化和矿化废物中，粗蛋白酶的蛋白质去除效率更高（95％），观察到了类似的趋势。同样，用5％NaOH处理粉末使脱矿和矿化废物中的蛋白质去除量分别从初始浓度0.3 mg / ml分别减少了约96％和87.6％。由？E产生的蛋白酶。 chrysanthemi？可用于将海鲜加工业中的废物转化为具有商业价值的产品。

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