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Optimization of epidemiological conditions to enhance the mycoherbicidal efficacy of Alternaria alternata against Chenopodium album

机译:优化流行病学条件以增强链格孢对白粉病菌的杀真菌功效

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A?new foliage disease was found on?Chenopodium album?L. (family chenopodiaceae) in Punjab, Pakistan.?Alternaria alternata?(Fr.) Keissler was identified and confirmed as the causal agent. The present study aimed to optimize environmental condition that could enhance mycoherbicidal potential of?A. alternataagainst?C. album. In growth room trials,?the effects of various inoculum concentrations of?A. ?alternata?(105, 107?and 109?conidia ml-1) on disease development was studied at different growth stages of the host plant (5 - 10, 10 - 15 and 20 - 25 leaf/flowering stage) at various dew period (100% humidity for 12, 18 and 24 h) and temperature (20, 25 and 30°C) regimes. To enhance the mycoherbicidal potential of the pathogens, different formulations; 1 and 2% gelatin, 1 and 2% carboxymethylcellulose (CMC), 1:1 gelatin and CMC, 10 and 20% canola oil emulsion were used. The pathogenicity of?A. alternata?increased with increasing spore concentration and length of dew period. A spore concentration of 109?conidia ml-1?in 20% canola oil emulsion with 24 h dew period and at temperature 25 and 30°C caused 100% mortality of?C. album?plants at 5 - 10 and 10 - 15 leaf stages and resulted in maximum reduction in biomass of the target weed. The present study concludes that under a certain set of conditions,?A. alternata?can completely control?C. album.
机译:在白屈菜(Chenopodium album)L上发现了新的叶子病。巴基斯坦旁遮普邦的chenopodiaceae科(家族)。Alternariaalternata(神父)被确定为致病菌。本研究旨在优化可增强?A杀真菌潜力的环境条件。对抗?专辑。在生长室试验中,不同接种浓度的A的影响。研究了在不同露水期的寄主植物不同生长阶段(5-10、10-15和15-20和25-25叶/开花期)对疾病的“ alternata”(105、107和109分生孢子ml-1)的影响。 (100%湿度持续12、18和24小时)和温度(20、25和30°C)制度。为了增强病原体的杀菌力,采用不同的配方;使用1%和2%的明胶,1%和2%的羧甲基纤维素(CMC),1:1明胶和CMC,10%和20%的低芥酸菜子油乳液。 A的致病性。交替孢子随着孢子浓度的增加和露水时间的延长而增加。 20%的低芥酸菜子油乳剂中,在24 h的露水温度下,在25和30°C的温度下,孢子浓度为109?conidia ml-1 ?,导致100%的死亡率。在5-10和10-15叶期种植白菜,从而最大程度地减少了目标杂草的生物量。本研究的结论是,在一定条件下,ΔA。交替链球菌可以完全控制吗?专辑。

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