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首页> 外文期刊>African Journal of Biotechnology >Real-time TaqMan polymerase chain reaction to quantify the effects of different sources of dietary starch on Bifidobacterium in the intestinal tract of piglets
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Real-time TaqMan polymerase chain reaction to quantify the effects of different sources of dietary starch on Bifidobacterium in the intestinal tract of piglets

机译：实时TaqMan聚合酶链反应以定量不同日粮淀粉来源对仔猪肠道双歧杆菌的影响

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Twenty-eight PIC male piglets (similar birth and parity, weaned at 21±1.5 days) were used to study the effect of?the different sources of dietary starch on the number ofBifidobacteria?in the digesta of the duodenum, jejunum, ileum, cecum and colon.Pigs were randomly assigned to one of four diets?formulated with corn starch, wheat starch, tapioca or pea starch. The determined ratio of amylose to amylopectin for these starches was 0.21, 0.24, 0.12 and 0.52, respectively?based on the 16s rRNA sequences of maximum species of?Bifidobacterium?from GenBank to design the primers and probe.?TaqMan polymerase chain reaction was developed to quantify the number of?Bifidobacterium.?We used this assay?to detect genomic DNA ofBifidobacterium?in the intestinal tract digesta of piglets, including duodenum, jejunum, ileum, cecum and colon.?Our?results indicated that,?developed new real-time quantitative PCR assays can?be?allowed for rapid, convenient, reproducible and steady quantification of the?Bifidobacterium?in the intestinal content of piglets. Additionally, the present study revealed that high amylose/amylopectin ratio of starches significantly enhanced the numbers of?Bifidobacterium?in all segments of intestine.
机译：用28只PIC雄性仔猪（成年和胎次相似，在21±1.5天断奶）研究不同饮食淀粉来源对十二指肠，空肠，回肠，盲肠消化道中双歧杆菌数量的影响。猪被随机分配到以玉米淀粉，小麦淀粉，木薯淀粉或豌豆淀粉配制的四种日粮中的一种。根据GenBank中双歧杆菌最大种类的16s rRNA序列设计引物和探针，确定这些淀粉的直链淀粉与支链淀粉之比分别为0.21、0.24、0.12和0.52。开发TaqMan聚合酶链反应。定量分析双歧杆菌的数量。我们使用此分析方法检测了仔猪肠道消化道（包括十二指肠，空肠，回肠，盲肠和结肠）中双歧杆菌的基因组DNA。我们的结果表明，开发了新的真可以进行实时定量PCR分析，以快速，方便，可重现和稳定地定量仔猪肠道内容物中的双歧杆菌。另外，本研究表明，淀粉的直链淀粉/支链淀粉的比例高，可显着提高肠道所有区段中双歧杆菌的数量。

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