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首页> 外文期刊>African Journal of Biotechnology >Inositol-P6 induced structural changes in duck major haemoglobin
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Inositol-P6 induced structural changes in duck major haemoglobin

机译：肌醇-P6诱导鸭主要血红蛋白的结构变化

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The reactivities of 5,5’-Dithiobis (2-nitrobenzoate) with the sulphydryl groups of major haemoglobin of duck (Anas platyrhinchos) have been carried out in the presence of inositol hexakisphosphate, inositol-P6. The time course of the reaction is biphasic. The addition of inositol-P6?changed the pH dependence profile of the apparent second-order rate constant of both oxy- and carbonmonoxyhaemoglobin for the reaction with CysF9(93)β. Inositol-P6?reduced the rate of reaction with CysF9(93)β of the carbonmonoxy derivative by about 2 to 9-fold, depending on the pH. In contrast,the?addition of inositol-P6?increased the rates of reaction of the oxy- and aquomet derivatives. This difference might have risen from the fact that the salt bridge that is normally formed in human haemoglobins between HisHC3(146)β and AspFG(94)β was not formed in the presence of inositol-P6?in liganded derivatives whose pH profiles were changed. Consequently, HisHC3(146)β comes sufficiently close to the CysF9(93)β sulphydryl group to interact with it electrostatically and so influences its reactivity directly. However, inositol-P6?changed only that of carbonmonoxyhaemoglobin for CysB5(23)β. For the reaction with CysB5(23)β, inositol-P6?reduced the rate in the carbonmonoxy derivative with increasing pH up to about 15-fold at pH 9.0, and increased the rates of reaction of the oxy- and aquomet derivatives by about 2 to 3-fold.
机译：5，5′-二硫代双（2-硝基苯甲酸酯）与鸭（Anas platyrhinchos）主要血红蛋白的巯基基团的反应是在肌醇六磷酸肌醇P6存在下进行的。反应的时间过程是两相的。肌醇-P6的添加改变了与CysF9（93）β反应的表观速率常数的氧和碳一氧血红蛋白的pH依赖性曲线。肌醇-P6降低与一氧化碳衍生物的CysF9（93）β反应的速率约2至9倍，具体取决于pH值。相反，肌醇-P6的添加增加了氧和水合衍生物的反应速率。这种差异可能是由于以下事实造成的：通常在人血红蛋白中HisHC3（146）β和AspFG（94）β之间形成的盐桥不是在肌醇-P6α存在的情况下在pH值已改变的配体衍生物中形成的。 。因此，HisHC3（146）β足够靠近CysF9（93）β巯基基团，可与其静电相互作用，因此直接影响其反应性。然而，肌醇-P6仅改变了CysB5（23）β的一氧化碳血红蛋白。对于与CysB5（23）β的反应，肌醇-P6？在pH 9.0时，随着pH值增加至约15倍，降低了一氧化碳衍生物的速率，并使氧和水合衍生物的反应速率提高了约2倍。至3倍。

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