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首页> 外文期刊>African Journal of Biotechnology >Direct and indirect plant regeneration from various explants of eastern cottonwood clones (Populus deltoides Bartram ex Marsh.) with tissue culture
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Direct and indirect plant regeneration from various explants of eastern cottonwood clones (Populus deltoides Bartram ex Marsh.) with tissue culture

机译：通过组织培养从东部三角叶杨克隆（Populus deltoides Bartram ex Marsh。）的各种外植体直接和间接再生植物。

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Populus?species are important resource for certain branches of industry and have special roles for scientific study on biological and agricultural systems. Plant regeneration via direct and indirect organogenesis of four?Populus?deltoidesBartram ex Marsh. ssp.?deltoides?×?Populus?deltoides?Bartram ex Marsh. ssp.deltoides?hybrid?clones?(89 M 011, 89 M 044, 89 M 048, 89 M 066)?and?P. deltoidesssp.?deltoides?clone (Samsun) were investigated. Direct organogenesis was established from nodes and internodes on woody plant medium (WPM) supplemented with cytokinins and/or auxins. The 89 M 011 clone gave the highest percentage (100%) of regeneration on WPM with 1 mg/l zeatine from internode explants. Indirect organogenesis via callus phase was obtained from nodes and petioles on WPM supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D). The nodes part of the 89 M 066 clone gave the highest rate of generative callus (100%) on WPM supplemented with 2 mg/l 2,4-D. Indirect shoots were obtained from the node callus on WPM with cytokinins. There was root formation from directly regenerative shoots which were cultured on WPM or Murashige and Skoog Basal Medium (MS) containing different ratios of indole butyric acid (IBA). Rooted seedlings?in vitro?were successfully acclimatized. Data on?in vitro?study were subjected to statistical evaluation. The?in vitro?regeneration system will allow this study to set reliable procedures for the genus and clones.
机译：胡杨树种是某些工业分支的重要资源，对生物和农业系统的科学研究具有特殊作用。通过四个PopulusdeltoidesBartram ex Marsh直接和间接器官发生的植物再生。 ssp.?deltoides?×?Populus?deltoides?Bartramex Marsh。 ssp.deltoides？hybrid？clones（89 M 011、89 M 044、89 M 048、89 M 066）和？研究了deltoidesssp.?deltoides？克隆（Samsun）。在补充了细胞分裂素和/或生长素的木本植物培养基（WPM）上，从节间和节间建立了直接器官发生。 89 M 011克隆从节间外植体中以1 mg / l的济汀在WPM上再生的百分比最高（100％）。通过愈伤组织阶段的间接器官发生是从补充了不同浓度的2,4-二氯苯氧基乙酸（2,4-D）的WPM上的节和叶柄获得的。 89 M 066克隆的节点部分在补充了2 mg / l 2,4-D的WPM上产生的愈伤组织比率最高（100％）。使用细胞分裂素从WPM的结节愈伤组织获得间接芽。直接再生芽有根形成，这些芽在含有不同比例的吲哚丁酸（IBA）的WPM或Murashige和Skoog基础培养基（MS）上培养。已成功地使生根幼苗适应环境。对体外研究的数据进行统计评估。体外再生系统将使这项研究为属和克隆建立可靠的程序。

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