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首页> 外文期刊>African Journal of Biotechnology >A probiotic bacterium, Pediococcus pentosaceus OZF, isolated from human breast milk produces pediocin AcH/PA-1
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A probiotic bacterium, Pediococcus pentosaceus OZF, isolated from human breast milk produces pediocin AcH/PA-1

机译：从人母乳中分离出的益生菌，戊糖戊球菌OZF产生了pediocin AcH / PA-1

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摘要

Pediococcus pentosaceus?OZF, originally isolated from healthy human breast milk, produces antimicrobial activities against many gram-positive bacterial species, including the food borne pathogen,?Listeria monocytogenes. A bacteriocin was purified to homogeneity from the supernatant of exponentially growing cells using ion-exchange and reversed-phase chromatography. The purification resulted in a high recovery and an approximately 224.000 fold increase in specific activity. The molecular mass of the bacteriocin was 4,623 Da as determined by electrospray ionization mass spectrometry.?Based on the DNA sequences of the pediocin AcH/PA-1 operon, specific primers were synthesized and used to determine whether the purified pediocin was identical to another previously reported bacteriocin. Results show that the bacteriocin produced by?P. pentosaceus?OZF was identical to pediocin AcH/PA-1. We thus refer to the identified bacteriocin as pediocin AcH/PA-1 in order to avoid confusion. The value of this report is that the producer strain is shown to be distinct from the pediocin PA-1 producer (P. pentosaceus?PA-1) based on a sugar fermentation profile.
机译：戊糖小球菌OZF最初从健康的人母乳中分离出来，对许多革兰氏阳性细菌，包括食源性病原体单核细胞增生李斯特菌，产生抗菌活性。使用离子交换和反相色谱从指数生长细胞的上清液中纯化细菌素至均质。纯化导致高回收率，比活性提高了约224.000倍。通过电喷雾电离质谱法测定，细菌素的分子量为4,623 Da。根据pediocin AcH / PA-1操纵子的DNA序列，合成了特异性引物，并用于确定纯化的pediocin是否与先前的另一个相同报道细菌素。结果表明？P产生了细菌素。戊糖纲？OZF与pediocin AcH / PA-1相同。因此，为了避免混淆，我们将鉴定出的细菌素称为pediocin AcH / PA-1。该报告的价值在于，根据糖发酵曲线，该生产菌株显示出与pediocin PA-1生产菌株（P. pentosaceus？PA-1）不同。

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