...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Novel SNPs polymorphism of bovine CACNA2D1 gene and their association with somatic cell score
【24h】

Novel SNPs polymorphism of bovine CACNA2D1 gene and their association with somatic cell score

机译：牛CACNA2D1基因的新型SNPs多态性及其与体细胞评分的关系

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mastitis is a major cause of economic loss in dairy cattle.?In this study, the bovineCACNA2D1?gene was taken as a candidate gene for mastitis resistance. The objective of this study was to identify single nucleotide polymorphisms (SNPs) in the bovine?CACNA2D1?gene and evaluate the association of these SNPs with mastitis in cattle. Through DNA sequencing and PCR-RFLP analysis, three mutations?C367400T, A496561G and G519663A?were detected in the cattle CACNA2D1 gene. Altogether 240 dairy cattle of three breeds (Holstein, Simmental, and Sanhe cattle) were genotyped and allele frequencies were determined. The effects of CACNA2D1 polymorphisms on somatic cell score (SCS) were analyzed and a significant association was found between?G519663A?and SCS. The mean of genotype GG was significantly lower than those of genotypes AG and AA.?The results of this?research will be useful in further studies to determine the role of the CACNA2D1 gene in mastitis resistance and?further work will be necessary to investigate whether the CACNA2D1 gene play a role in defending the host from mastitis.
机译：乳腺炎是造成奶牛经济损失的主要原因。在这项研究中，牛CACNA2D1α基因被认为是抵抗乳腺炎的候选基因。本研究的目的是鉴定牛？CACNA2D1？基因中的单核苷酸多态性（SNP），并评估这些SNP与牛乳腺炎的关系。通过DNA测序和PCR-RFLP分析，在牛CACNA2D1基因中检测到三个突变：C367400T，A496561G和G519663A。对三个品种（荷斯坦，西门塔尔牛和三河牛）的240头奶牛进行了基因分型，并确定了等位基因频率。分析了CACNA2D1多态性对体细胞评分（SCS）的影响，并发现G519663A与SCS之间存在显着关联。 GG基因型的平均值显着低于AG和AA基因型。这项研究的结果对于进一步研究确定CACNA2D1基因在乳腺炎抗性中的作用将是有用的，并且有必要进一步研究CACNA2D1基因在保护宿主免受乳腺炎的侵害中发挥作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号