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Evaluation of the antioxidant and antityrosinase activities of three extracts from Pleurotus nebrodensis fruiting bodies

机译:白灵菇子实体三种提取物抗氧化和抗酪氨酸酶活性的评价

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Pleurotus nebrodensis?has been widely used for nutritional and medicinal purposes. The aim of the present study was to evaluate the antioxidant activities and tyrosinase inhibitory effects on the fruiting bodies of?P.?nebrodensis?extracted with acetone, methanol and hot water. The antioxidant activities were performed on β-carotene-linoleic acid, reducing power, 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferrous ions chelating abilities and xanthine oxidase inhibitory activity. In addition to this, phenolic compounds were also detected. The acetonic and methanolic extracts of?P.?nebrodensis?showed the strongest β-carotene-linoleic acid inhibition as compare to hot water extract. At 8 mg/ml, the acetonic extract showed a high reducing power of 1.86. The scavenging activity on DPPH radicals, the acetonic and methanolic extracts were effective than that of hot water extract. At lower concentrations, chelating effect of tested mushroom was significantly effective as compare to positive control.?Gallic acid, protocatechuic acid, chlorogenic acid, ferulic acid, naringenin, hesperetin, formononetin and biochanin-A were detected from acetonitrile and hydrochloric acid solvent extract. The xanthine oxidase andtyrosinase inhibitory activities of the acetonic, methanolic and hot water extracts increased with increase of concentration. This study suggests that fruiting bodies ofP.?nebrodensis?can potentially be used as a readily accessible source of natural antioxidants.
机译:杏鲍菇已被广泛用于营养和药用目的。本研究的目的是评估丙酮,甲醇和热水提取的对虾松节肢动物的子实体的抗氧化活性和酪氨酸酶抑制作用。对β-胡萝卜素-亚油酸,还原能力,1,1-二苯基-2-吡啶并肼基(DPPH),亚铁离子螯合能力和黄嘌呤氧化酶抑制活性进行了抗氧化活性。除此之外,还检测到酚类化合物。与热水提取物相比,“ P.nebrodensis”的丙酮和甲醇提取物显示出最强的β-胡萝卜素-亚油酸抑制作用。在8 mg / ml时,乙炔提取物显示出1.86的高还原力。对DPPH自由基,丙酮和甲醇提取物的清除活性比热水提取物有效。在较低的浓度下,与阳性对照相比,测试蘑菇的螯合效果显着有效。?从乙腈和盐酸溶剂提取物中检测出了苹果酸,原儿茶酸,绿原酸,阿魏酸,柚皮苷,橙皮苷,formononetin和biochanin-A。丙酮,甲醇和热水提取物的黄嘌呤氧化酶和酪氨酸酶抑制活性随浓度的增加而增加。这项研究表明,P。?nebrodensis?的子实体可以潜在地用作容易获得的天然抗氧化剂来源。

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