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The effect of NaCl on proline metabolism in Saussurea amara seedlings

机译:NaCl对雪莲幼苗脯氨酸代谢的影响。

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Many plants accumulates proline dramatically under a variety of stress conditions, and this play an important role in regulation of osmotic homeostasis, prevention of damages caused by osmotic stresses, scavenging of reactive oxygen species and protection of cell structures. The regulation of proline accumulation in?Saussurea amara?seedlings and the activities of key enzymes involved in proline metabolism in response to salinity were studied, and 50-days-old seedlings were treated with NaCl(0, 100, 200, 300 and 400 mM) in this experiment. The results showed that proline contents were higher in leaves than in roots and increased significantly with NaCl concentration and treatment duration?(P < 0.05).?The activity of?pyrroline-5-carboxylate synthetase (P5CS)?increased at first and than decreased with increaso of NaCl concentration. The activities of?orn-δ-aminotransferase (δ-OAT) and proline dehydrogenase (ProDH) increased and decreased, respectively, in response to elevated NaCl concentration?and treatment duration. The data demonstrated that glutamate and ornthine pathway were activated by NaCl, and proline biosynthesis mainly depended on glutamate pathway under low-concentration NaCl condition, in which P5CS took dominant position. Conversely, the ornthine pathway was predominant.
机译:许多植物在各种胁迫条件下会大量积累脯氨酸,这在调节渗透稳态,防止渗透胁迫引起的破坏,清除活性氧和保护细胞结构方面发挥重要作用。研究了雪莲(Saussurea amara)幼苗中脯氨酸积累的调控以及盐胁迫下脯氨酸代谢所涉及的关键酶的活性,并用NaCl(0、100、200、300和400 mM处理了50天大的幼苗)。 )。结果表明,叶片中脯氨酸的含量高于根部,并随NaCl浓度和处理时间的延长而显着增加(P <0.05)。吡咯啉-5-羧酸合成酶(P5CS)的活性先升高后降低。随着氯化钠浓度的增加。随着NaCl浓度的升高和处理时间的延长,α-δ-氨基转移酶(δ-OAT)和脯氨酸脱氢酶(ProDH)的活性分别增加和减少。数据表明,谷氨酸和鸟氨酸途径被NaCl激活,脯氨酸的生物合成主要依赖于低浓度NaCl条件下的谷氨酸途径,其中P5CS占据主导地位。相反,鸟氨酸途径占主导。

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