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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity in 14 tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets by RAPD-PCR technique
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Genetic diversity in 14 tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets by RAPD-PCR technique

机译：应用RAPD-PCR技术研究尼日利亚市场上14个番茄品种的遗传多样性

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摘要

A total of 226 tomato (Lycopersicon?esculentum?Mill.) fruits sold in markets within three states in Southwestern Nigeria were purchased and differentiated into 14 varietal groups based on morphological parameters. The genomic DNA from the young apical leaves of the seeded plants were extracted and analyzed by the randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique. Seventy-four?(74)?amplified products were scored with 62.2% of them revealing polymorphism. The OPB-18 primer recorded the highest polymorphism (83.3%) and OPU-14 the least (44.4%). The dendrogram revealed 3 clusters. The genotypic relationships between varieties within clusters ranged from 2 in clusters 1 and 3 to 10 in cluster 2. Cluster 2 revealed 4 subgroups with two pairs of varieties (var. c?and?i and l?and?m) showing 100% similarities. The local names, place of cultivation and phenotypic characters expressed by the varieties were not suitable for varietal differentiation since phenotypically different fruits showed genotypic relatedness. The impression that tomato varieties from a particular geographical zone are genetically similar was therefore nullified.
机译：总共购买了在尼日利亚西南部三个州内的市场上出售的226个番茄（Lycopersicon？esculentum？Mill。）水果，并根据形态参数将其分为14个品种组。通过随机扩增的多态性DNA-聚合酶链反应（RAPD-PCR）技术提取并分析了种子植物幼嫩叶的基因组DNA。对74（74）个扩增产物进行打分，其中62.2％显示多态性。 OPB-18引物记录的多态性最高（83.3％），而OPU-14记录的最低（44.4％）。树状图显示3个簇。聚类中品种之间的基因型关系从聚类1中的2和聚类2中的10到聚类2中。聚类2揭示了4个亚组，其中有两对变种（var。c？and？i和l？and？m）显示出100％的相似性。该品种表达的当地名称，栽培地点和表型特征不适合用于品种分化，因为表型不同的果实表现出基因型相关性。因此消除了来自特定地理区域的番茄品种在遗传上相似的印象。

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