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首页> 外文期刊>African Journal of Biotechnology >Fine structure of Egyptian buffalo oocytes (Bubalus bubalis) during different in vitro maturation periods using transmission electron microscopy (TEM)
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Fine structure of Egyptian buffalo oocytes (Bubalus bubalis) during different in vitro maturation periods using transmission electron microscopy (TEM)

机译：使用透射电子显微镜（TEM）在不同的体外成熟期，埃及水牛卵母细胞（Bubalus bubalis）的精细结构

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摘要

This study was carried out to illustrate the ultrastructure of Egyptian buffalo oocytes through?in vitro?maturation (IVM) period (24 h). Selected cumulus oocytes complexes (COCs) were divided into three groups in relation to the time of maturation (8, 16 and 24 h >). After different maturation intervals, the matured oocytes were examined by light and transmission electron microscope (TEM). Results showed that the cumulus cells were close to each other and zona plucida (ZP) in the first group than the second and third group, lipid droplets (LD) appeared normal and nearly from plasma membrane in the group of oocytes matured?in vitro?for 8 h than oocytes matured?in vitro?for 24 h, microvilli (Mv) appeared with more number, higher thickness and penetrating the ZP and cytoplasm in the first group than other two groups, and mitochondria (M) in the first group were located near the plasma membrane, while in the third group it appeared as individual and scattered through the oocyte cell. Finally, the first polar body was observed in the third group only. This study showed the ultra-structural concepts of Egyptian buffalo oocytes during different IVM periods, and provided insights about the changes that occurred in oocytes during maturation process in the bovine species.
机译：这项研究的目的是通过体外成熟期（IVM）（24 h）阐明埃及水牛卵母细胞的超微结构。相对于成熟时间（8、16和24 h>），将选定的卵母细胞复合物（COC）分为三类。在不同的成熟间隔后，通过光和透射电子显微镜（TEM）检查成熟的卵母细胞。结果表明，在第一组卵丘细胞与第二组和第三组卵透明带（Zona plucida）之间彼此接近，在体外成熟的卵母细胞组中脂滴（LD）呈正常状态，且接近于质膜。与体外培养的卵母细胞相比，在体外培养24h后的8h中，第一组的微绒毛（Mv）出现数量更多，厚度更大，并穿透ZP和细胞质，而第一组的线粒体（M）位于质膜附近，而在第三组中，它表现为单个并分散在卵母细胞中。最后，仅在第三组中观察到第一极体。这项研究显示了在不同的IVM时期埃及水牛卵母细胞的超微结构概念，并提供了关于牛物种成熟过程中卵母细胞发生变化的见解。

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