• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Isolation of AhDHNs from Arachis hypogaea L. and evaluation of AhDHNs expression under exogenous abscisic acid (ABA) and water stress
【24h】

Isolation of AhDHNs from Arachis hypogaea L. and evaluation of AhDHNs expression under exogenous abscisic acid (ABA) and water stress

机译：外来脱落酸和水分胁迫下花生花生AhDHNs的分离及表达的评价

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The peanut?(Arachis hypogaea?L.)?is an important oil and cash crop all over the world. It is mostly planted in arid and semi-arid regions. To determine the mechanism by which dehydrins (DHNs) are regulated by abscisic acid (ABA) in peanuts, three?Arachis hypogaea?L. dehydrins?(AhDHNs) were isolated from peanut plants and sequenced. By blasting the protein sequences of these?AhDHNs,?AhDHN1?was found belonging to the YnSKn subfamily.?AhDHN2?and?AhDHN3?were found belonging to the SKn and YnKn types, respectively. 100 μM ABA enhanced?AhDHNs?expression in peanut leaves. When peanut plants were treated with ABA and then with the ABA synthesis inhibitor sodium tungstate 12 h later,?AhDHN?expression was suppressed. However,?AhDHN2was inhibited by sodium tungstate at 2 h, though other?AhDHNs?were not.?AhDHNsexpressions increased greatly in peanut leaves treated with 30% polyethylene glycol (PEG). Sodium tungstate along with PEG inhibited the expression of?AhDHNs. This study found that exogenous and endogenous ABA can both affect the expression ofAhDHN?independently. The differential expression of?AhDHNs?to exogenous ABA may be because of differences in the structure of different?AhDHNs.
机译：花生（Arachis hypogaea？L。）是全世界重要的石油和经济作物。它主要种植在干旱和半干旱地区。为了确定花生中脱落酸（ABA）调节脱水蛋白（DHNs）的机制，需要三个花生。从花生植株中分离出脱水蛋白（AhDHNs）并进行测序。通过对这些ΔAhDHNs的蛋白质序列进行扩增，发现ΔAhDHN1′属于YnSKn亚家族。ΔAhDHN2′和ΔAhDHN3′分别属于SKn和YnKn类型。 100μMABA增强了花生叶片中AhDHNs的表达。当花生植物先用ABA处理，再用ABA合成抑制剂钨酸钠处理12小时后，αAhDHNα的表达被抑制。然而，钨酸钠在2 h抑制了AhDHN2的表达，而其他AhDHNs没有受到抑制。用30％聚乙二醇（PEG）处理的花生叶片中，AhDHN的表达大大增加。钨酸钠与PEG一起抑制αAhDHNs的表达。这项研究发现外源和内源性ABA都可以独立地影响AhDHNα的表达。 ΔAhDHNs与外源ABA的差异表达可能是由于不同ΔAhDHNs的结构差异所致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号