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Biochemical response and host-pathogen relation of stalk rot fungi in early stages of maize (Zea mays L.)

机译:玉米早期腐烂真菌的生化反应和宿主-病原体关系

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Stalk rot is a destructive disease in maize caused by?Fusarium and?Macrophominaspecies. A study was carried out to understand the mode of infection, host biochemical response and comparison of inoculation techniques in?Fusarium verticillioides?and?Macrophomina phaseolina?in maize. In seed inoculation experiment, high mycelia growth on seed surface lead to rotting in 36.6% of seeds inoculated by?F. verticillioides?and 10.0% seeds in?M. phaseolina.?In seedling inoculation experiment, twenty one days old seedlings raised in glasshouse were inoculated with spore suspension of both pathogens, respectively in two sets, resulting in symptoms like tip drying, necrotic lesions, chlorotic bands, pale green leaves and yellowing of margins in varying numbers. Significant result was the appearance of asymptomatic seedlings in?F. verticillioides?infection which was confirmed by the increase in total soluble phenols (9.39 mg/g) and total sugars (5.33 mg/g) content in comparison to?the?control (2.84 mg/g?total soluble solid (TSS)?and 2.18 mg/g total soluble phenols) and symptomatic ones. While in?M. phaseolina,?total contents of sugar and soluble phenols were on part in asymptomatic and control (uninfected), depicting disease escape to be the possible cause of this phenotypic expression. The study concludes that inoculation techniques for screening of genotypes play a major role. The appearance and non appearance of symptoms in infected host can mislead the identification of resistant genotypes.
机译:秸秆腐烂是玉米中的一种由镰刀菌和Macrophomina物种引起的破坏性疾病。进行了一项研究,以了解玉米中“ Fusarium v​​erticillioides”和“ Macrophomina phaseolina”的感染方式,宿主生化反应以及接种技术的比较。在种子接种实验中,种子表面高菌丝体生长导致?F接种的36.6%种子腐烂。丝状麦角菌和10.0%的种子。菜苗接种实验中,在温室中培育的21天大的幼苗分别接种了两种病原体的孢子悬浮液,分为两组,导致叶尖干燥,坏死性病变,褪绿带,浅绿色的叶子和边缘发黄。数量不等。显着的结果是在?F中出现了无症状的幼苗。与总对照固体(TSS)相比(2.84 mg / g),总可溶性酚(9.39 mg / g)和总糖(5.33 mg / g)含量的增加证实了黄萎病菌的感染。 2.18 mg / g总可溶性酚)和有症状的酚。在吗?菜豆中的糖和可溶性酚的总含量部分无症状和未受控制(未感染),说明疾病逃逸可能是这种表型表达的可能原因。该研究得出结论,用于筛选基因型的接种技术起着重要作用。感染宿主中症状的出现和不出现可能会误导耐药基因型的鉴定。

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