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首页> 外文期刊>African Journal of Biotechnology >Isolation of potent biosurfactant producing bacteria from oil spilled marine water and marine sediments
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Isolation of potent biosurfactant producing bacteria from oil spilled marine water and marine sediments

机译：从溢油的海水和海洋沉积物中分离出有效的生物表面活性剂产生细菌

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Biosurfactants produced by bacteria are surface-active compounds involved in the degradation of hydrocarbons. They are heterogeneous group of surface active molecules produced by microorganisms, which adhere to cell surface or are excreted extracellularly in the growth medium. These can be divided into low- molecular weight molecules, that lower surface and interfacial tensions efficiently and high-molecular-weight polymers that bind tightly to surfaces. These surfactants, produced by a wide variety of microorganisms, have very different chemical structures and surface properties. It is therefore reasonable to assume that different groups of biosurfactants have different natural roles in the growth of the producing microorganisms. In this study, isolation and identification of biosurfactant producing bacteria were assessed from oil-spilled seawater collected from harbors and docks from Arabian Sea (Mumbai), India. The potential application of these bacteria in microbial enhanced oil recovery (MEOR) was investigated. To confirm the ability of isolates in biosurfactant production, various biosurfactant activity assay tests and measurement of surface tension were conducted. Hemolysis was used as a criterion for the primary isolation of biosurfactant producing bacteria. Effects of different pH, salinity and temperature on biosurfactant production were also studied. Among all of the isolated strains,Pseudomonas?sp.?(MW2) showed high salt tolerance, successful production of biosurfactant in a vast pH and temperature domain and reduced surface tension of the medium to value below 40 mN/m. This strain is a potential candidate for MEOR. The MW2 biosurfactant component was detected as glycolipid in nature.
机译：细菌产生的生物表面活性剂是参与烃降解的表面活性化合物。它们是由微生物产生的表面活性分子的异质基团，其粘附于细胞表面或在生长培养基中被细胞外排泄。这些可分为低分子量分子（可有效降低表面张力和界面张力）和高分子量聚合物（可紧密结合至表面）。由多种微生物产生的这些表面活性剂具有非常不同的化学结构和表面性质。因此，可以合理地假设不同组的生物表面活性剂在生产微生物的生长中具有不同的自然作用。在这项研究中，从从印度阿拉伯海（孟买）的港口和码头收集的溢油海水中评估了产生生物表面活性剂细菌的分离和鉴定。研究了这些细菌在微生物增油中的潜在应用（MEOR）。为了确认分离物在生物表面活性剂生产中的能力，进行了各种生物表面活性剂活性测定试验和表面张力测量。溶血被用作初步分离产生生物表面活性剂的细菌的标准。还研究了不同pH，盐度和温度对生物表面活性剂生产的影响。在所有分离的菌株中，假单胞菌（Pseudomonas？sp。）（MW2）显示出高耐盐性，在广泛的pH和温度范围内成功生产了生物表面活性剂，并将介质的表面张力降低至40 mN / m以下。该菌株是MEOR的潜在候选者。 MW2生物表面活性剂成分在自然界中被检测为糖脂。

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