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首页> 外文期刊>African Journal of Biotechnology >Optimization of the Clustal X program for the alignment of multicopper oxidase bacterial sequences and their presence in the Bacillus genus
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Optimization of the Clustal X program for the alignment of multicopper oxidase bacterial sequences and their presence in the Bacillus genus

机译：Clustal X程序的优化，用于比对铜氧化酶细菌序列及其在芽孢杆菌属中的存在

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Bioinformatic methods for the alignment of amino acid sequences and function prediction have to be robust, principally in conserved regions bound to enzymatic activity. In order to optimize amino acid sequence alignment in multicopper oxidase conserved sites of a collection of 103 bacterial sequences, modification were made in the?weight values?in different matrices?for amino acid residues?from the Clustal X 1.83 program. The results obtained with modified Clustal X program allowed us to identify different multicopper oxidase activity according to the groups from the relationship diagram, identifying two well differentiated groups within the?Bacillus?genus. This analysis allowed infers multicopper oxidase activity in?Bacillus thuringiensis?similar to that presented by?Bacillus halodurans, but distinct with respect to other laccase type bacterial oxidases. Specific oligonucleotides were designed to amplify through polymerase chain reaction (PCR) the gene that codifies for the?B. thuringiensislaccase, finding that this sequence is present in only 6 out of 32 sero varieties of?B. thuringiensis?analyzed.
机译：氨基酸序列比对和功能预测的生物信息学方法必须稳健，主要是在与酶活性结合的保守区内。为了优化在103个细菌序列的多铜氧化酶保守位点的氨基酸序列比对，从Clustal X 1.83程序修改了“不同基质中氨基酸残基的权重值”。修改后的Clustal X程序获得的结果使我们能够根据关系图从各组中识别出不同的多铜氧化酶活性，并鉴定了芽孢杆菌属中的两个高分化组。这种分析可以推断苏云金芽孢杆菌中的多铜氧化酶活性类似于嗜盐芽孢杆菌所呈现的活性，但与其他漆酶型细菌氧化酶不同。设计特定的寡核苷酸，以通过聚合酶链反应（PCR）扩增编码βB的基因。苏云金漆酶，发现该序列仅存在于32个B血清变种中的6个。苏云金芽孢杆菌？

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