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Stability of Spodoptera litura nucleopolyhedrovirus in sodium dodecyl sulphate

机译:斜纹夜蛾核多角体病毒在十二烷基硫酸钠中的稳定性

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Sodium dodecyl sulfate (SDS) is a foaming agent used to extract occlusion bodies (OBs) of baculoviruses from infected cells or tissues of an insect. However, these OBs may be damaged by SDS at certain concentrations. In this study, the impact of different concentrations of SDS on OBs of?Spodoptera litura?nucleopolyhedrovirus (SpltNPV) was determined. Even though the number of OBs harvested increased with the increase in the concentration of SDS used in the extraction, the percentage of damaged OBs also increased correspondingly as detected through scanning electron microscope (SEM) photomicrographs. The result also indicates that infectivity of SpltNPV on second instars of?S. litura?larvae reduced significantly with an increase of SDS concentration used in the treatment. Among the four concentrations of SDS tested, SDS at 0.1% was able to release more quality virus. Thus, SDS of less than 0.1% is recommended for obtaining optimum number of quality OBs.
机译:十二烷基硫酸钠(SDS)是一种发泡剂,用于从昆虫的感染细胞或组织中提取杆状病毒的闭塞体(OB)。但是,某些浓度的SDS可能会损坏这些OB。在这项研究中,确定了不同浓度的SDS对斜纹夜蛾核多角体病毒(SpltNPV)OB的影响。即使通过提取中使用的SDS浓度的增加,收获的OB的数量增加,但通过扫描电子显微镜(SEM)显微照片检测到,受损的OB的百分比也相应增加。该结果还表明SpltNPV对ΔS的第二龄期的感染性。随着处理中SDS浓度的增加,荔枝幼虫显着减少。在所测试的四种浓度的SDS中,0.1%的SDS能够释放出更高质量的病毒。因此,建议使用小于0.1%的SDS以获得最佳数量的质量OB。

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