• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Cloning, structural analysis and expression of cardiac troponin C (TNNC1) gene in goat
【24h】

Cloning, structural analysis and expression of cardiac troponin C (TNNC1) gene in goat

机译：山羊心肌肌钙蛋白C（TNNC1）基因的克隆，结构分析及表达

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cardiac troponin (TNNC1) is the calcium-binding subunit of the myofibril thin filament that regulates excitation-contraction coupling in cardiac muscle. In the study, we cloned?TNNC1?gene of goat for the first time (GenBank accession number: HQ640744) and analyzed its tissue expression. Results indicate that TNNC1 was a 161-amino acid polypeptide that had been highly conserved during evolution. Its nucleotide sequence was high and was similar in various animals ranging from 97.94 (cow) to 83.33% (African clawed frog), and the identity of the encoded amino acids varied from 100 (cow) to 92.55% (smelt). Fluorescence quantitative PCR analyses revealed that the?TNNC1?gene was selectively expressed in the muscular tissues of goat, was expressed in cardiac tissue and slow skeletal muscle (soleus), but was not expressed in fast skeletal muscle (longissimus muscle, gluteus maximus) and brain, kidney, lung or liver of goat. The amino acid residue of Ca2+?binding site II is identical in mammals, aves and fish. This study may provide more insight into the molecular structure, expression patterns and evolution of?TNNC1?gene in animal.
机译：心肌肌钙蛋白（TNNC1）是肌原纤维细丝的钙结合亚基，调节心肌中的兴奋收缩耦合。在这项研究中，我们首次克隆了山羊的“ TNNC1”基因（GenBank登录号：HQ640744）并分析了其组织表达。结果表明TNNC1是一种161个氨基酸的多肽，在进化过程中一直高度保守。其核苷酸序列很高，并且在各种动物中相似，范围从97.94（牛）到83.33％（非洲爪蛙），编码氨基酸的身份从100（牛）到92.55％（熔炼）不等。荧光定量PCR分析表明，TNNC1基因在山羊肌肉组织中选择性表达，在心脏组织和慢速骨骼肌（比目鱼肌）中表达，而在快速骨骼肌中（长背肌，臀大肌）不表达。山羊的脑，肾，肺或肝。 Ca2 +β结合位点II的氨基酸残基在哺乳动物，鸟类和鱼类中是相同的。这项研究可以为动物中TNNC1基因的分子结构，表达模式和进化提供更多的见识。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号