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首页> 外文期刊>African Journal of Biotechnology >In vitro mecA gene transfer among Staphylococcus aureus in Malaysian clinical isolates
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In vitro mecA gene transfer among Staphylococcus aureus in Malaysian clinical isolates

机译：马来西亚临床分离株中金黄色葡萄球菌之间的体外mecA基因转移

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摘要

A simple experiment was performed to observe?in vitro?transfer of?mecA?gene from methicillin-resistant?Staphylococcus aureus,?Malaysian clinical isolates, to methicillin-sensitive one. PCR-based method was used in combination with the selective antibiotic screening method to study the transfer of resistance among clinical isolates carrying different genetic markers. After performing the mixed liquid culture experiments, two types of the transconjugants were obtained. Transconjugants were analyzed by PCR for the presence of?mecA?and?ccrrecombinase genes. The?mecA,?ccrA3?and?ccrB2?genes were detected in donor strains as well as in both types of transconjugants. Transfer of?mecA,?ccrA3?andccrB2?genes from donor to recipient strains that were present in close proximity during?in vitro?experiment may suggest possibility of horizontal gene transfer (HGT) among?S. aureus?clinical strains. Bacterial co-infections?in vivo?where the bacteria could be in close proximity are likely to be a possible point of horizontal gene transfer (HGT) with regard to certain antibiotic resistant genes.
机译：进行了一个简单的实验，以观察“ mecA”基因从耐甲氧西林的金黄色葡萄球菌（马来西亚临床分离株）向甲氧西林敏感的菌株的“体外”转移。基于PCR的方法与选择性抗生素筛选方法结合使用，以研究耐药性在带有不同遗传标记的临床分离株之间的转移。在进行混合液体培养实验之后，获得了两种类型的转缀合物。通过PCR分析转导结合体中是否存在“ mecA”和“ ccr重组酶”基因。在供体菌株和两种转导结合体中都检测到了“ mecA”，“ ccrA3”和“ ccrB2”基因。 mecA，？ccrA3？和ccrB2？基因从供体转移到在体外实验过程中紧密相邻的受体菌株中，可能提示在？S之间进行水平基因转移（HGT）的可能性。金黄色葡萄球菌临床菌株。对于某些抗生素抗性基因，细菌可能在体内的细菌共感染（可能在细菌附近）可能是水平基因转移（HGT）的可能点。

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