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首页> 外文期刊>African Journal of Biotechnology >Reproducible and expedient rice regeneration system using in vitro grown plants
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Reproducible and expedient rice regeneration system using in vitro grown plants

机译：使用体外生长的植物可再生且方便的水稻再生系统

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Inevitable prerequisite for expedient regeneration in rice is the selection of totipotent explant and developing an apposite combination of growth hormones. Here, we reported a reproducible regeneration protocol in which basal segments of the stem of the?in vitro?grown rice plants were used as ex-plant. Using the protocol, callus was developed from the exposed cells of root segments and cortical tissues of basal part of the stem. Various levels of 2,4-dichlorophenoxyacetic acid (2,4-D) were used, where 1 mg/L was found as best level for callus induction. Further, 25 combinations of kinetin and naphthalene acetic acid (NAA) were developed to investigate the regeneration response of the calli. The root-derived calli did not respond to any combination at all, whereas the nodular calli derived from the stem segments responded variably to kinetin and NAA combinations in the Murashige and Skoog (MS) medium from base to top. Higher kinetin to NAA ratios promoted embryogenesis, whereas lower ratios exhibited rhizogenesis. A combination of 3 mg/L kinetin and 1 mg/L NAA was established to be the best combination for plant regeneration through embryogenesis in rice from?in vitro?grown plants. Plants regenerated?in vitro?were successfully acclimatized in the pots, where they exhibited phenotypically indistinguishable normal growth when compared with plants developed from seed, hence the developed regeneration system for rice in these studies may be treated as one of the best strategies to?in vitro?clonal propagation and purification, ahead of seasonal growth of plants in the field or green house.
机译：水稻中方便再生的必然前提是选择全能外植体并开发适当的生长激素组合。在这里，我们报道了一种可再生的再生方案，其中将体外生长的水稻植株茎的基础部分用作离体植株。使用该协议，从根节的裸露细胞和茎基部的皮质组织发育出愈伤组织。使用了各种水平的2,4-二氯苯氧基乙酸（2,4-D），其中1 mg / L被认为是诱导愈伤组织的最佳水平。此外，开发了激动素和萘乙酸（NAA）的25种组合以研究愈伤组织的再生反应。根源的愈伤组织完全不响应任何组合，而在Murashige和Skoog（MS）培养基中，从茎段衍生的结节性愈伤组织对激动素和NAA组合的反应从底部到顶部均不同。较高的激动素与NAA比例促进了胚发生，而较低的比例则显示了发根。建立3 mg / L激动素和1 mg / L NAA的组合是通过离体生长的植物在水稻中通过胚发生进行植物再生的最佳组合。体外再生的植物已成功地适应了盆栽，与从种子发育的植物相比，它们表现出表型上无法区分的正常生长，因此，在这些研究中，发达的水稻再生系统可被视为实现水稻再生的最佳策略之一。在田间或温室植物季节性生长之前进行体外克隆繁殖和纯化。

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