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首页> 外文期刊>African Journal of Biotechnology >Analysis of genetic diversity in female, male and half sibs willow genotypes through RAPD and SSR markers
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Analysis of genetic diversity in female, male and half sibs willow genotypes through RAPD and SSR markers

机译：通过RAPD和SSR标记分析雌性，雄性和半同胞杨柳基因型的遗传多样性

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Willows belong to the genus?Salix?(Salicaceae) and consist of large number of species with large phenotypic variations. As a result, it has a low diagnostic value for identifying pure species and interspecific hybrids. Genetic characterization of 34 reference genotypes (4 female, 10 male, and 20 half sibs) of?Salix?collected from Naganji Nursery of University of Horticulture and Forestry, Solan, Himachal Pradesh, India were analyzed using 10 SSRs and 15 RAPDs PCR-based molecular markers. RAPD analysis yielded 87 polymorphic fragments (98.9%), with an average of 5.8 polymorphic fragments per primer. Similarly, SSR analysis produced 33 bands, out of which 26 were polymorphic (78.8%) with an average of 2.6 polymorphic fragments per primer. The genetic diversity was high among the genotypes (Nei’s genetic diversity = 0.468 and Shannon’s information index = 0.659) as measured by combination of both RAPD and SSR markers. The mean coefficient of gene differentiation (Gst) was 0.034, indicating 96.6% of the genetic diversity resided within the genotypes. The genetic diversity among genotypes of?Salix?sp. was found to be high, suggesting the importance and feasibility of introducing elite genotypes from different origins for?Salixgermplasm conservation and breeding programs.
机译：柳树属于Salix科（Salicaceae），由大量具有较大表型变异的物种组成。结果，它对于鉴定纯种和种间杂种具有低诊断价值。使用10个SSR和15个基于PCR的PCR分析了印度喜马拉雅州索兰市园艺与林业大学Naganji苗圃收集的Salix的34种参考基因型（4名女性，10名男性和20个半同胞）的遗传特征。分子标记。 RAPD分析得到87个多态性片段（98.9％），每个引物平均5.8个多态性片段。同样，SSR分析产生了33个条带，其中26个是多态性的（78.8％），每个引物平均有2.6个多态性片段。根据RAPD和SSR标记的组合，这些基因型的遗传多样性很高（Nei的遗传多样性= 0.468，Shannon的信息指数= 0.659）。基因分化的平均系数（Gst）为0.034，表明96.6％的遗传多样性存在于基因型中。萨利克斯氏菌基因型之间的遗传多样性。被发现具有很高的遗传率，这表明引进不同来源的优良基因型对于种质资源保存和育种计划的重要性和可行性。

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