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首页> 外文期刊>African Journal of Biotechnology >Tissue culture as an alternative for commercial corm production in saffron: A heritage crop of Kashmir
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Tissue culture as an alternative for commercial corm production in saffron: A heritage crop of Kashmir

机译：组织培养替代藏红花商业球茎的生产：克什米尔的传统农作物

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摘要

The present study aimed at developing a commercially viable protocol for?in vitro?corm production in saffron. Three-step sterilization process involving fungicides and sterilants ensured 94% clean viable cultures.?Plant growth regulator?(PGRs) ensuring initial bud sprouting, direct shoot regeneration from the base of the sprouted bud and cormlet production from multiple shoots have been standardized. MS Media supplemented with 0.5 mg/l naphthalene acetic acid (NAA) and 1.5 mg/l 6-benzyl amino purine (BAP) ensured maximum bud sprouting in September with direct multiple shoot primordia initiation on 6.5 mg/l BAP in November. 6.5 mg/l BAP + 0.2 mg/l NAA resulted in maximum shoot proliferation (24); however, at higher concentration, the PGRs were detrimental in arresting the growth. Viable shoot clumps established maximum?in vitro?corms in April after sub culturing on growth retardant (CCC) at 0.25% supplemented with 9% sucrose. Subculturing of non flowering?in vitro?corms on growth retardant with sucrose eliminated season dependence of?in vitro?protocols in the 2nd?cycle of protocol. Primary and secondary hardening before field transfer ensured 100% corm viability.
机译：本研究旨在开发藏红花中体外球茎生产的商业可行方案。由杀菌剂和杀菌剂组成的三步灭菌过程确保了94％的清洁可行培养。植物生长调节剂（PGRs）确保初始芽萌芽，从芽芽基部直接芽再生以及从多个芽中产生cor子标准化。 MS Media补充有0.5 mg / l的萘乙酸（NAA）和1.5 mg / l的6-苄基氨基嘌呤（BAP），确保了9月的最大芽萌芽，并于11月以6.5 mg / l的BAP直接引发多芽原基。 6.5 mg / l BAP + 0.2 mg / l NAA导致最大的芽增殖（24）;然而，在较高浓度下，PGRs对阻止生长有害。在0.25％的生长延缓剂（CCC）上补充9％的蔗糖进行继代培养后，4月份有活力的芽丛建立了最大的体外球茎。用蔗糖将非开花的体外球茎传代培养在生长延缓剂上，消除了协议第二个周期中体外协议的季节依赖性。现场转移之前的初次和二次硬化确保了100％的球茎生存能力。

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