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首页> 外文期刊>African Journal of Biotechnology >In-vitro propagation of Picralima nitida (Stapf) through embryo culture
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In-vitro propagation of Picralima nitida (Stapf) through embryo culture

机译：通过胚胎培养体外培养Picralima nitida（Stapf）

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摘要

Embryo abortion in wide crosses and seed dormancy has hampered the mass propagation of selected tree germplasm from the wild. An?in vitro?plant regeneration protocol was successfully established in?Picralima nitida?(Stapf), a medicinal tropical plant, by culturing excised embryo from mature seeds collected from the wild. Seeds were sterilized and dissected; embryos were raised?in vitro?in 11 different media compositions comprising pure Murashige and Skoog (MS) and MS media supplemented with varying concentrations of cytokinins labeled A, B, C, D, E, F, G, H, I, J and K. Media type was significant on metrical traits of sprouts (P ≤ 0.05). One hundred percent survival of explants were obtained in media J while MS basal medium (K) had the lowest survival of 40%; and mean number of leaves (1.4). Number of roots per sprout, shoot and root length responses were lowest in medium H with values of 0.13, 0.79 and 0.15 cm, respectively. Overall best growth parameters in shoot (3.38 cm) and root lengths (3.75 cm) were obtained in medium F. Mass propagation of?P. nitida?can be accomplished in MS media supplemented with 0.05 mg/L kinetin and 0.01 mg/L naphthalene acetic acid (NAA).?Key word:?Excised embryo,?In vitro?culture, sprouts, metrical traits,?Picralima nitida.
机译：广泛流传的胚胎流产和种子休眠已阻碍了野生树木资源的大量繁殖。通过从野外采集的成熟种子中培养出离体胚胎，成功地在药用热带植物尼加尔瓜（Picralima nitida）（Stapf）中建立了一种体外植物再生方案。将种子消毒并解剖;在11种不同的培养基组合物中（包括纯的Murashige和Skoog（MS））以及添加了不同浓度的细胞分裂素的A，B，C，D，E，F，G，H，I，J和K.培养基类型对豆芽的性状特征具有重要意义（P≤0.05）。在培养基J中外植体的存活率为100％，而MS基础培养基（K）的最低存活率为40％;平均叶数（1.4）。在H培养基中，每芽的根数，枝条和根长响应最低，分别为0.13、0.79和0.15 cm。在培养基F中获得了茎（3.38 cm）和根长（3.75 cm）的总体最佳生长参数。ΔP的大量繁殖。 nitida可在补充有0.05 mg / L激动素和0.01 mg / L萘乙酸（NAA）的MS培养基中完成。关键词：离体胚胎;离体培养;芽苗，度量特征; Picralima nitida。

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