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首页> 外文期刊>African Journal of Biotechnology >Production of -amylase from some thermophilic Aspergillus species and optimization of its culture medium and enzyme activity
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Production of -amylase from some thermophilic Aspergillus species and optimization of its culture medium and enzyme activity

机译：从某些嗜热曲霉中生产α-淀粉酶及其培养基和酶活性的优化

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摘要

This paper aimed to evaluate the thermophilic and hyperthermophilic fungi isolated from extreme conditions capable of secreting α-amylase, an enzyme that has a commercial value especially in the production of bread in the food industry and also to ensure their optimization. In this study, thermostable amylase activities of some thermophilic Aspergillus species were evaluated. The suitable medium and microorganisms for α-amylase synthesis were selected. Subsequently, the α-amylase activity of the microorganism was researched. In the measurements made on the 7th day of production on respectively Aspergillus niger, Aspergillus oryzae, Aspergillus terreus cultures produced at mycological, stock basal medium (SBM) and starch yeast extract liquid medium in order to determine a more efficient medium for amylase activity, the α-activity observed was 1.83, 1.66 and 0.75 U/ml in the mycological liquid medium, 2.37, 2.28 and 1.69 U/ml in the SBM medium and 6.8, 5.9 and 5.1 U/ml in the starch yeast extract liquid medium. The optimum temperature (65°C) and the optimum pH 8, respectively, and activity of α-amylase enzyme obtained from A. niger showed the highest activity level.
机译：本文旨在评估从能够分泌α-淀粉酶的极端条件下分离的嗜热和超嗜热真菌，α-淀粉酶是一种具有商业价值的酶，特别是在食品工业中的面包生产中，并确保其优化。在这项研究中，评估了一些嗜热曲霉属物种的热稳定淀粉酶活性。选择适合α-淀粉酶合成的培养基和微生物。随后，研究了微生物的α-淀粉酶活性。在生产的第7天，分别对黑曲霉，米曲霉，米曲霉培养物进行生产，分别在真菌学，基础基础培养基（SBM）和淀粉酵母提取液培养基上进行测定，以确定更有效的淀粉酶活性培养基。在真菌学液体培养基中观察到的α活性为1.83、1.66和0.75 U / ml，在SBM培养基中观察到的α-活性为2.37、2.28和1.69 U / ml，在淀粉酵母提取物液体培养基中为6.8、5.9和5.1 U / ml。从黑曲霉获得的最适温度（65℃）和最适pH 8，以及α-淀粉酶的活性显示出最高的活性水平。

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