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首页> 外文期刊>African Journal of Biotechnology >Improved embryogenic callus induction and plant regeneration in big bluestem (Andropogon gerardii Vitman), a potential bioenergy feedstock
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Improved embryogenic callus induction and plant regeneration in big bluestem (Andropogon gerardii Vitman), a potential bioenergy feedstock

机译：大蓝茎（Andropogon gerardii Vitman）（一种潜在的生物能源原料）中改善了胚性愈伤组织的诱导和植物再生

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The objective of this study was to develop an efficient regeneration protocol in big bluestem, a potential feedstock that produces huge biomass. Embryogenic calli were induced from the seeds of cultivars, Kaw and Earl, on Murashige and Skoog (MS) medium with different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) (0.2, 0.5, 1.0, 2.0, 3.0 and 5.0 mg l-1) alone or in combination with 6-benzylaminopurine (BA) (0.5 mg l-1) or L-proline (2.0 g l-1). In Kaw, the highest number of embryogenic calli (39.1%) was induced on MS + 0.5 mg l-1 2,4-D and L-proline, whereas in Earl, the highest number of embryogenic calli (39.8%) was obtained on MS medium containing 1.0 mg l-1 2,4-D and L-proline. The embryogenic calli were then transferred to regeneration media (MS medium supplemented with kinetin, 0.2, 0.5, 1.0, 3.0 and 5.0 mg l-1 or BA, 0.2, 0.5, 1.0, 3.0 and 5.0 mg l-1). Shoots were regenerated on all of the concentrations tested and the regeneration percentage and number of shoots per calli increased with the increase in BA or kinetin concentration. Regenerated shoots were transferred to half strength MS medium for rooting. The fully developed plantlets were established in the greenhouse. The regeneration protocol established in this study may be used for the application of genetic engineering technologies in big bluestem.
机译：这项研究的目的是在大蓝茎（一种可能产生大量生物质的原料）中开发有效的再生方案。在不同浓度的2,4-二氯苯氧基乙酸（2,4-D）（0.2、0.5、1.0、2.0、3.0和3.0）的Murashige和Skoog（MS）培养基上，从品种Kaw和Earl的种子中诱导出胚性愈伤组织。 5.0 mg l-1）单独或与6-苄氨基嘌呤（BA）（0.5 mg l-1）或L-脯氨酸（2.0 g l-1）组合使用。在Kaw中，MS + 0.5 mg l-1 2,4-D和L-脯氨酸诱导的胚性愈伤组织数量最高（39.1％），而在Earl中，经MS + 0.5 mg l-1 2,4-D和L-脯氨酸诱导的胚性愈伤组织数量最多。 MS培养基，含1.0 mg -1-1 2,4-D和L-脯氨酸。然后将胚发生的愈伤组织转移至再生培养基（补充了激动素0.2、0.5、1.0、3.0和5.0mg l-1或BA，0.2、0.5、1.0、3.0和5.0mg l-1的MS培养基）。芽在所有测试浓度下均再生，并且随着BA或激动素浓度的增加，再生率和每愈伤组织的芽数增加。将再生的芽转移到半强度MS培养基中生根。在温室中建立了完全发育的苗。本研究建立的再生方案可用于大蓝茎基因工程技术的应用。

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