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首页> 外文期刊>African Journal of Biotechnology >Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)
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Somatic embryogenesis from zygotic embryos and thin cell layers (TCLs) of Brazilian oil palm (Elaeis guineensis Elaeis oleifera)

机译：来自巴西油棕（Elaeis guineensis Elaeis oleifera）的合子胚和薄细胞层（TCL）的体细胞胚发生

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摘要

Oil palm hybrid BRS Manicoré is important for plantations in the north of Brazil, as it is resistant to fatal yellowing and is compact. Seed germination is slow and reduced, so somatic embryogenesis is a promising alternative for its propagation. Two kinds of starting explants were used: Zygotic embryos (ZE) and thin cell layers (TCL) from the base of seedling aerial parts. Two culture medium formulae were compared (Y3 and modified MS), and several growth regulators (2,4-D, Picloram, BA, 2iP), putrescine and activated charcoal (AC) were used during the different stages. Somatic embryos (SE) were obtained from ZE-derived embryogenic masses cultured on Y3 medium with or without 2,4-D (9 μM) + 1000 μM putrescine and 40% were converted into seedlings in the Y3 medium supplemented with 2 g.L-1 AC and without growth regulators, under light. SE developed in 49 to 53% TCL-derived calli and 50% of them were converted into seedlings in the same conditions described for ZE calli. The yield of both processes was compared, showing the superiority of TCL explants for SE production.
机译：油棕杂交种BRSManicoré对巴西北部的人工林非常重要，因为它可以抵抗致命的泛黄并且结构紧凑。种子发芽缓慢且减少，因此体细胞胚发生是其繁殖的有希望的替代方法。使用了两种起始外植体：合子胚（ZE）和来自幼苗空中部分底部的薄细胞层（TCL）。比较了两种培养基配方（Y3和改良的MS），并在不同阶段使用了几种生长调节剂（2,4-D，Picloram，BA，2iP），腐胺和活性炭（AC）。体细胞胚（SE）是从ZE衍生的胚发生物质中获得的，该胚发生物质在带有或不带有2,4-D（9μM）+ 1000μM腐胺的Y3培养基上培养，并在补充有2 gL-1的Y3培养基中转化为幼苗交流电，无生长调节剂，光照下。 SE在49％至53％TCL衍生的愈伤组织中发育，其中50％在与ZE愈伤组织相同的条件下转化为幼苗。比较了两种方法的产率，显示了TCL外植体在SE生产中的优越性。

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