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首页> 外文期刊>African Journal of Biotechnology >Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)
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Comparison between Giemsa and Van Geison stains in demonstration of collagen fibers (Kosti-2016)

机译：Giemsa和Van Geison染色剂在胶原蛋白纤维演示中的比较（Kosti-2016）

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摘要

Trichrome stain (such as Van Geison) is usually used in histopathology laboratory for demonstration of collagenic fibers. Lack of selectivity and tendency of stain to fade makes van Gieson not ideal for collagen demonstration. This study was aimed to compare between Giemsa's and van Gieson's stains in collagen fibers demonstration. Twenty biopsies were obtained from rabbit’s skin after anesthesia by chloroform and immediately fixed by 10% neutral buffered formalin for 48 h. Then samples were processed using tissue processing machine and sectioned by rotary microtome. Two hundred (200) tissue sections of 5 micron thickness were prepared. A 100 tissue sections was stained by Van Geison and another 100 tissue sections stained by Giemsa. The stained section was compared with illustrated photomicrographs in order to assess staining quality. Best collagen staining quality was obtained by Van Geison's 60 (60%) and 40 (40%), mean 1.40, followed by Giemsa's stain excellent 55 (55%) and good 45 (45%), mean 1.45. Conclusively, Van Geison's is superior but Giemsa stain is rapid, sensitive without fading tendency, easy to perform and low cost and can be used as special stain under optimized conditions.
机译：三色染色剂（例如Van Geison）通常在组织病理学实验室中用于演示胶原纤维。缺乏选择性和污渍掉色的趋势使van Gieson不适用于胶原蛋白展示。这项研究旨在比较吉姆萨氏染色和范吉森氏染色在胶原纤维演示中的染色。麻醉后用氯仿从兔子的皮肤中取出20份活检样品，并立即用10％中性福尔马林缓冲液固定48小时。然后使用组织处理机处理样品并通过旋转切片机进行切片。准备了两百（200）个5微米厚的组织切片。 Van Geison将100个组织切片染色，而Giemsa将另外100个组织切片染色。将染色的部分与图示的显微照片进行比较，以评估染色质量。 Van Geison的60（60％）和40（40％）（平均1.40）获得最佳的胶原蛋白染色质量，其次是Giemsa的55（55％）和45（45％）好的染色（平均1.45）。总之，Van Geison染色剂性能优越，但Giemsa染色剂快速，敏感，无褪色趋势，易于操作且成本低廉，可在优化条件下用作特殊染色剂。

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