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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity in two populations of Limicolaria aurora (Jay, 1839) from two ecological zones in Nigeria
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Genetic diversity in two populations of Limicolaria aurora (Jay, 1839) from two ecological zones in Nigeria

机译：尼日利亚两个生态区的两个极光Limicolaria aurora（Jay，1839）种群的遗传多样性

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Limicolaria aurora belongs to the group of land snails commonly called garden snails. This study seeks to use shell morphology and random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) to examine gross morphological differences in populations of L. aurora from New Bussa (guinea savannah) and Benin City (tropical rain forest) in Nigeria and possibly delimit the populations into sub species. A total of one hundred and ten specimens of L. aurora made up of fifty five individuals from each of the two ecological zones were collected randomly for the study. Data on shell parameters including: height of shell (SH), width of shell (SW), aperture height (AH), aperture width (AW), spire length (SL), and first whorl length (1WL) measured on each snail were subjected to one way analysis of variance (ANOVA). Principal component analysis (PCA) and canonical variates analysis (CVA) were performed on the data using PAST statistical software. DNA which was extracted from the muscular tissues of the foot of eight individuals from each location using cetyltrimethylammonium bromide (CTAB) method was subjected to RAPD-PCR. Amplification of the DNA was done using five primers (OPB-12, OPB-18, OPH-08, OPD-11 and OPS-13). Analyses showed significant differences (P<0.05) in L. aurora populations within and between the vegetation zones revealing great heterogeneity in the populations. Both PCA and CVA clusters did not separate the populations into distinct sub-populations. SH was the most variable morphological characteristic and consequently the most suitable for the separation of L. aurora specimens into distinct populations. All the primers used in the amplification of the DNA produced polymorphic bands. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster diagram revealed two major clades within the snail populations with about 74% similarity. The study showed that RAPD-PCR analysis is more suitable for delimiting populations of L. aurora than morphometrics and that the basis for gross morphological differences in these populations might not only be environmental but also genetic factors.
机译：极光冬青属属于蜗牛类，通常称为花园蜗牛。这项研究旨在使用壳形态学和随机扩增多态性DNA聚合酶链反应（RAPD-PCR）来研究尼日利亚和墨西哥的新布莎（几内亚热带草原）和贝宁市（热带雨林）的极光乳杆菌种群的总体形态差异。可能将种群划分为亚种。随机收集了来自两个生态区中每一个的一百五十个由五十五个人组成的极光乳杆菌样本。分别在每只蜗牛上测得的壳参数数据包括：壳高（SH），壳宽（SW），孔径高度（AH），孔径宽度（AW），塔尖长度（SL）和第一螺纹长度（1WL）接受方差单向分析（ANOVA）。使用PAST统计软件对数据进行主成分分析（PCA）和规范变量分析（CVA）。使用十六烷基三甲基溴化铵（CTAB）从每个位置从八个人的脚的肌肉组织提取的DNA进行RAPD-PCR。使用五种引物（OPB-12，OPB-18，OPH-08，OPD-11和OPS-13）进行DNA扩增。分析表明，在植被带内和之间的极光乳杆菌种群存在显着差异（P <0.05），表明种群之间存在很大的异质性。 PCA和CVA群集均未将种群分为不同的亚群。 SH是最易变的形态特征，因此最适合将极光乳杆菌标本分离成不同的种群。用于DNA扩增的所有引物产生多态性条带。带有算术平均值的非加权对群方法（UPGMA）聚类图显示了蜗牛种群中的两个主要进化枝，相似度约为74％。该研究表明，RAPD-PCR分析比形态计量学更适合于界定极光乳杆菌种群，这些种群中总体形态差异的基础可能不仅是环境因素，而且还包括遗传因素。

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