• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites
【24h】

Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites

机译：微卫星揭示的绿色常绿高粱及其衍生物的遗传多样性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The genetic variability of 28 sorghum genotypes of known senescence phenotype was investigated using 66 SSR markers well-distributed across the sorghum genome. The genotypes of a number of lines from breeding programmes for stay-green were also determined. This included lines selected phenotypically for stay-green and also RSG 03123, a marker-assisted backcross progeny of R16 (recurrent parent) and B35 (stay-green donor). A total of 419 alleles were detected with a mean of 6.2 per locus. The number of alleles ranged from one for Xtxp94 to 14 for Xtxp88. Chromosome SBI-10 had the highest mean number of alleles (8.33), while SBI-05 had the lowest (4.17). The PIC values obtained ranged from zero to 0.89 in Xtxp94 and Xtxp88, respectively, with a mean of 0.68. On a chromosome basis, mean PIC values were highest in SBI-10 (0.81) and lowest in SBI-05 (0.53). Most of the alleles from B35 in RSG 03123 were found on chromosomes SBI-01, SBI-02 and SBI-03, confirming the successful introgression of quantitative trait loci associated with stay-green from B35 into the senescent background R16. However, the alternative stay-green genetic sources were found to be distinct based on either all the SSRs employed or using only those associated with the stay-green trait in B35. Therefore, the physiological and biochemical basis of each stay-green source should be evaluated in order to enhance the understanding of the functioning of the trait in the various backgrounds. These genetic sources of stay-green could provide a valuable resource for improving this trait in sorghum breeding programmes.Keywords: Simple sequence repeats, sorghum, stay-green, genetic diversity.
机译：使用在整个高粱基因组中分布良好的66个SSR标记，研究了28种已知衰老表型的高粱基因型的遗传变异性。还确定了用于保持绿色的育种程序中许多系的基因型。这包括在表型上为保持绿色选择的品系，以及RSG 03123，R16（轮回亲本）和B35（保持绿色的供体）的标记辅助回交后代。总共检测到419个等位基因，平均每个位点6.2个。等位基因的数量范围从Xtxp94的一个到Xtxp88的14。染色体SBI-10的等位基因平均数最高（8.33），而SBI-05的最低等位基因数（4.17）。在Xtxp94和Xtxp88中，获得的PIC值分别为零至0.89，平均值为0.68。以染色体为基础，平均PIC值在SBI-10中最高（0.81），在SBI-05中最低（0.53）。 RSG 03123中大多数来自B35的等位基因位于SBI-01，SBI-02和SBI-03染色体上，证实了与来自B35的常绿相关的定量性状基因座已成功渗入衰老背景R16。然而，基于使用的所有SSR或仅使用与B35中的保持绿色性状相关的SSR，发现了保持绿色的替代遗传资源是不同的。因此，应评估每种保持绿色来源的生理和生化基础，以增强对不同背景下性状功能的理解。这些保持绿色的遗传资源可以为提高高粱育种程序的性状提供有价值的资源。关键词：简单序列重复，高粱，保持绿色，遗传多样性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号